EECENT METHODS FOR THE DIAGNOSIS OF GLANDERS. 
157 
loses its power of agglutination, due to the decrease in the activity 
of the agglutinins. 
Case No. 12 gave no reaction to the agglutination test, but 
did to the two mallein tests. The mare was kept under obser¬ 
vation until taken to a neighboring town, up to which time no 
clinical symptoms were shown. 
Case No. 15 will be noticed to have given a decided, positive 
reaction with this method, although the temperature chart ob¬ 
tained from the injection with mallein was unsatisfactory, on ac¬ 
count of the impossibility of making either a positive or negative 
interpretation, due to the rather high pre-injection temperature. 
This animal would have been considered suspicious under the 
first mallein test, but the agglutination test clinched the diagnosis. 
This was further verified by the appearance, shortly afterward, of 
clinical symptoms in the form of enlarged submaxillary lymph 
glands. Some six weeks later the swelling* had nearly disap¬ 
peared and the mare seemed to be enjoying good health. The 
second agglutination and mallein tests were both positive. Un¬ 
doubted clinical symptoms appeared within a few weeks. Post¬ 
mortem examination revealed well-marked pulmonary lesions. 
For ordinary clinical use we have found this method of diag¬ 
nosis very desirable, and thus sustain the experience of other 
veterinarians. The results are particularly reliable in cases of 
recent infection; chronic cases may occasionally give an agglu¬ 
tination value lower than that of a healthy horse. 
REFERENCES. 
x. Konew, D. Prazipitation reaktion als diagnostische Methode beim Rotz. Cen- 
tralblatt f. Bakteriologle. Abt. i, Orig., Band 55, Heft 3, p. 251. July 9, 1909. 
2. Mohler, J. R. The Diagnosis of Glanders by the Precipitation Reaction of Konew. 
American Veterinary Review, Vol. XXXVIII, No. 4, January, 1911, p. 518. 
3. Moore, Taylor and Giltner. The Agglutination Method for the Diagnosis of 
Glanders. Proceedings of the American Veterinary Medical Association, 43d Annual 
Convention, 1906, p. 194. 
The Shawano County (Wis.) Journal , of April 4, announces 
the choice of Veterinarian B. Royer, as Mayor. We are sure the 
veterinarians of his city and state extend to him their hearty 
congratulations, as does the Review. 
