42S 
D. J. MANGAN. 
hours, during that time give it an occasional vigorous shaking. 
Then place the mixture in a Berkefield filter (No. 3-N-Med). 
The filtrate contains only the toxic material of the bacteria 
and diseased tissues in solution. Our text books tell us that most 
micro-organisms are endotoxic in character, but the use of the 
filter shows that the 'bacteria in the pus mixture permits a large 
quantity of their toxins to escape. 
One c.c. to 2.5 c.c. is the dose to be injected in the above 
described regions, and to be repeated according to the indications 
and progress of the case. 
The last two methods are according to Dr. Duncan’s instruc¬ 
tions and are the two I would suggest to be adopted. The first 
method is unreliable unless great care is exercised in its prepara¬ 
tion. If it receives too much heat it is useless and two little heat 
it is dangerous. Possibly in a water bath for about two hours at 
a temperature of 60 degrees C. may be a good way to prepare the 
pus for injecting. 
A dose of the autogenous antigen may be followed by a mild 
“ negative phase ” lasting hardly over 24 hours to be followed in 
turn by a “ positive phase ” extending over several days. A 
proper sized dose will be followed by the transitory “ negative 
phase ” and then the “ positive phase,’’ which may last for several 
days. 
The lesions so to speak are flushed with blood and an increased 
flow of lymph during this moderate “ negative phase ” when 
these substances carry a greater quantity of the protective agents 
of the body fluids, hence healing results. 
A dose of autogenous antigen may do damage to an organism 
that has experienced numerous autoinoculations and is in a con¬ 
dition of constant “ negative phase.” If administered at all, only 
in the smallest doses, well diluted, may any help be hoped for in 
such cases. 
Vaccines aid only in cases which have the necessary power 
to react to their stimulus. 
In a hyperplastic area surrounding a suppurating focus, or 
located in a joint the opsonins and anti-bacterial substances in the 
