K. A. WHITING. 
■45S 
Those receiving B. coli communis became sick, but recovered. 
Those receiving the bacterium became sick and died in from 
12 to 16 hours. An exposure check died in 36 hours. The 
lesions observed were those of septicaemia. A pure culture of 
the organism was recovered from the heart blood of the inocu¬ 
lated rabbits and injected into chickens, producing death in from 
28 to 48 hours. The lesions present in these experimental ani¬ 
mals were also characteristic of septicaemia hemorrhagica. Pul¬ 
lets that were inoculated subcutaneously with a pure culture of 
the bacterium from the hearts of the chicken and turkey died in 
from 16 to 21 hours, with lesions of hemorrhagic septicaemia. 
A pure culture of the bacterium was recovered from the hearts 
of the inoculated pullets and injected subcutaneously into old 
hens, producing death in from 22 to 36 hours. Mixed cultures 
of the bacterium and B. coli communis were then injected sub¬ 
cutaneously into an old hen, producing death in less than 16 
hours. A young pullet exposed to the inoculated birds died in 
6 days. A hen exposed under the same conditions remained 
well. 
The cultures used varied in age from the fourth to the twelfth 
generations and were grown at a temperature of 37 degrees C. 
The subcutaneous injections consisted of doses of 1 cc. of a 
24-hour bouillon culture. The virulence of the bacterium for 
chickens was apparently decreased to some extent after passage 
through the rabbit and to some extent after passage through 
fowls, and the virulence seemed to be increased by the addition 
of B. coli communis to the bacterium. Such a mixed culture 
produced death in from 12 to 16 hours even in old individuals, 
were identical both morphologically and culturally with the cul¬ 
tures first isolated from the hearts of the original chicken and 
turkey. 
The symptoms in the rabbits consisted of uneasiness, fever, 
drowsiness and coma. The pullets and hens first appeared stupid, 
later becoming drowsy with loss of appetite. Diarrhoea then set 
in, at first slight, gradually becoming more profuse, watery and 
of yellowish color. As soon as the diarrhoea began the birds 
