G5S 
B. F. KAUPP. 
methylene blue, when long thread-like metachromatic staining 
threads simulating Bacillus necrophorus were observed. Char¬ 
acteristic cultures were obtained in tubes of glucose agar and 
grown after the shake anaerobic culture method. 
A rabbit was inoculated subcutaneously and died on the sixth 
day. Upon removing the skin there was found characteristic 
necrosis with odor of necrobacillosis. Smears from this lesion 
stained and examined under the microscope showed the char¬ 
acteristic Bacillus necrophorus. Pure cultures were obtained as 
before. 
This case showed marked improvement under hot bichloride 
packs. 
Recently an autopsy was held on an eleven-month-old colt 
in the writer’s investigation work of “ no name ” disease in 
Southeastern Colorado 1 . An ulcer was observed in the stomach 
near the cardio-pyloric juncture and on the pyloric side. The 
ulcer measured one-half by three-fourth inch and was covered 
with a crust-like diphtheritic membrane like that observed in 
stomach ulcers in hogs, due to the Bacillus necrophorus. As a 
matter of routine the section was cut out and placed in formalin 
with the balance of the specimens. Upon sectioning, staining and 
studying this specimen under the microscope the part was found 
to be packed with bundles of the Bacillus necrophorus. 
Necrobacillosis of Cattle —It has been stated by some investi¬ 
gators that contagious ano-vulvitis so common among cows and 
heifers some years ago and which has again caused some trouble 
during the past year, is due to the Bacillus necrophorus. Necro¬ 
bacillosis among cattle is a serious disease in some parts of the 
country. In the San Louis Valley in Colorado much loss is 
realized. Recently there was sent to the writer’s laboratory the 
head of a calf from Palmer Lake. Ulcers due to the Bacillus 
necrophorus were found on both sides of the mouth, causing a 
bulging appearance from the outside. Upon examination there 
was found a sloughing of the mucous membranes covering the 
gums and cheek and had invaded the bone. This man had lost 
six calves out of eight. He stated that the condition appeared 
