152 
R. KOCH. 
29. —Scrofulous gland cultivated for six months in seven successive breed¬ 
ings ; 
30. —Scrofulous gland cultivated for five months in seven successive breed¬ 
ings ; 
31. —Scrofulous gland cultivated for three months in three successive breed¬ 
ings; 
32. —Scrofulous gland cultivated for three months in four successive breed¬ 
ings; 
33. —Tuberculous testicles cultivated for four months in six successive breed¬ 
ings : 
34. —Fungous joint cultivated for fifteen months in nineteen successive breed¬ 
ings ; 
35. —Lupus cultivated for sixteen months in twenty-one successive breedings ; 
36. —Lung from cattle-tuberculosis (caseous mass) cultivated for six months 
in eight successive breedings ; • 
37. —Lung from domestic animal tuberculosis (calcined knots) cultivated for 
five months in seven successive breedings ; 
38. —Knots from the diaphragm of a tuberculously diseased domestic animal 
cultivated for nine months in fifteen successive breedings; 
39. —Knots from the pericardium of a tuberculously diseased domestic animal 
cultivated for eighteen months in twenty-three successive breedings ; 
40. —Caseous pneumonia of the pig cultivated for five months in eight success¬ 
ive breedings; 
41. —Spontaneous tuberculosis of guinea pig (knots from the lungs) cultivated 
for six months in nine successive breedings; 
42. —Spontaneous tuberculosis of the guinea pig (spleen) cultivated for three 
months in five successive breedings ; 
43. —Spontaneous tuberculosis of the guinea pig (knots from the lung) culti¬ 
vated for four months in seven successive breedings. 
The preservation of the culturen demands such an expenditure of time and 
trouble, that always only a certain number can be maintained at the same time. 
I let the most of them perish again as soon as their vegetative disease-producing 
qualities had been sufficiently established by a “ culture ” continued for several 
months, and by the inoculations undertaken with it. Only the Culturen No. 1 
(lung-phthisis), No. 16 (tuberculosis of a domestic animal), No. 22 (miliary tuber¬ 
culosis), No. 26 (contents of a cavity from a phthisical lung). No. 34 (fungous joint), 
No. 35 (lupus), No. 39 (tuberculosis of a domestic animal) have been continued 
until now, and are to be further preserved in order lo find out whether in the life 
of the tuberculous bacilli continued outside of the animal body some sort of changes 
may not occur in their qualities. It might appear strange that comparatively so 
large a number of “culturen” were established, when a few would have sufficed 
for observing the conduct of the bacilli in the “ culturen.” Nevertheless in the 
beginning it appeared to me by no means impossible, that although the bacilli of 
the various tuberculous forms,—lupus, phthisis etc.—showed microscopically no 
difference, the bacilli derived from the various sources might perhaps manifest 
differences in the culturen. But in spite of the closest attention directed to this 
point, I have been able to find nothing of the kind. In the “culturen” also, 
