i60 
R. KOCH. 
responding with this the tuberculosis ran its course much more slowly. Never¬ 
theless also in these animals, the swelling, the first noticeable symptom of 
disease, and the later caseous degeneration of the inguinal glands, leave no doubt 
that the place of inoculation formed the point of entrance for the tuberculous 
virus. Four of the animals died from the tenth to the twelfth week, the others 
were then killed. In all the lymph glands in the neighborhood of the place of 
inoculation were caseous, and the spleen, liver and lungs tuberculous to a marked 
degree. 
19. — Scrofulous gland. —The gland substance, poor in bacilli, transferred into 
the anterior eye chamber of four rabbits. In all four animals in the course of the 
third week, tuberculosis of the iris began to develop and lead to caseous degener¬ 
ation of the bulbus. In the tenth week the rabbits were killed and beside the 
destruction of the bulbus, caseous degeneration of the neck lymph-glands and 
numerous grey knots were found in the lungs. 
20. —From five different cases of lupus eighteen rabbits were inoculated in 
the anterior eye-chamber. The course of the disease corresponded exactly with 
that described in No. 19. An iris-tuberculosis at first developing slowly, grad¬ 
ually leading to caseous degeneration and suppuration of the bulbus and finally 
to general tuberculosis. The inoculation was without results in the case of one of 
the rabbits. Some were killed just when the iris tuberculosis had developed, 
others after the swelling and caseous degeneration of the neck-glands had ap¬ 
peared; still others finally died with wide-spread tuberculosis of the lung, liver, 
spleen and kidneys. As well in the tubercles of the iris as in the tuberculously 
altered glands, lungs, etc., tuberculous bacilli were proved more or less abun¬ 
dantly. From a sixth lupus case three guinea pigs, and from one of the above 
mentioned cases five guinea pigs were subcutaneously inoculated. In these 
animals also there were swelling and caseous degeneration of the inguinal glands. 
They died in the seventh to the eighth week after the inoculation, were tubercu¬ 
lous in a high degree, and had numerous tuberculous bacilli* in the lungs, spleen, 
liver and kidneys. 
21. Lung affected by “perlsucht” partially calcareous knots with quite 
numerous bacilli, inoculated into eight guinea pigs. These died within five to 
eight weeks and were all tuberculous in a high degree. From one of these guinea 
pigs four others and from a second three others were inoculated. Of these ani¬ 
mals also five died in the sixth and seventh week, the last two were killed in the 
eighth week. In all these also tuberculosis was found. Further: From the 
“perlsucht” lung used in these attempts, a cat was inoculated and died after seven 
weeks, tuberculous. A second cat inoculated with lung tubercles from this ani¬ 
mal after six weeks appeared emaciated and short-breathed. She was killed and 
found to have numerous tubercles in the lungs and spleen. 
22. — A “ perlsucht ” knot from the peritoneum inoculated into six guinea 
^Lately Demme, Pfeifer and Dontrelepont have made communications relating to the 
occurrence of tuberculous bacilli in lupus-skin and in the tubercles of animals inoculated 
with lupus. My investigations in regard to lupus, which include not only the proof of ba¬ 
cilli in lupus skin and in inoculation tubercles, but also long continued “ rein-culturen ” of 
lupus-bacilli and successful inoculation undertaken with them, had been concluded for 
several months, when those communications were published, so that these could have had 
no influence upon my work. 
