164 
R. KOCH. 
were killed after thirty-four days the inoculated showed themselves tuberculous, 
the uninoculated healthy. 
Fourth experiment: Reincultur from the tuberculous lung of amonkey (No. 
11), cultivated 113 days in nine successive breedings, subcutaneously inoculated 
into two guinea pigs, one German marmot, six white rats, five white mice, four 
field mice, two hedge hogs, six domestic fowls, four doves, two sparrows, three 
eels, one goldfish, five frogs, one turtle. Of these animals only the guinea pigs, 
the marmot and the field mice became noticeably sick. These were killed fifty- 
three days after the inoculation and all found tuberculous to a high degree. The 
tuberculosis of the marmot has, according to all appearance, a very great resemb¬ 
lance to that of the guinea pig. The spleen is very much enlarged and has a grey¬ 
ish-red marbled appearance, also the liver appears permeated by large yellowish 
herds. The tuberculously changed organs of the field mouse also look very charac¬ 
teristic. The inguinal glands are considerably enlarged and caseous, the lungs 
permeated by numerous grey knots from the size of a poppy-seed to the head of 
a pin, and liver and spleen permeated very uniformly with many whitish tubercles 
as large as a grain of millet, so that these latter gained a very dainty sprinkled 
appearance. All the other animals of this experiment were killed two months 
later and it appeared in their investigation, that one of the five white mice had 
some grey knots in the lungs, the others were healthy, as were also the rats and 
the hedge hog. Of the domestic fowls, three had the large tuberculous knots in 
the intestines and in the liver characteristic of this species of animals. The rest 
of the animals were healthy. 
Fifth experiment: Reincultur from the closed vomica of a phthisic lung (No. 
26), cultivated for twelve months in sixteen successive breedings and subcutan¬ 
eously inoculated into seventeen guinea pigs, two other animals remaining un¬ 
inoculated. With these animals observations were made as to the effect of 
means which have the power of hindering the development of the tuberculous 
bacilli and they could therefore not be killed. In spite of the fact that partly 
arsenic, partly carbolic acid had been used to the greatest possible extent, the tu¬ 
berculosis ran its course just the same as in the former animals, the lymph-glands 
swelled considerably, emaciation occurred, all the animals died in the fourth to 
the sixth week and were tuberculous in a high degree. The two uninoculated 
animals were then killed and found healthy. 
Sixth experiment: The following reinculturen, first, from lupus (No. 35) in 
eight successive breedings c ontinued for five months; second, from a fungous 
joint (No. 34) in seven successive breedings for four months; third, from a scrof¬ 
ulous gland (No. 29) in seven successive breedings for five months ; fourth, from, 
miliary tuberculosis, (No. 22) in twelve successive breedings for nine months; 
fifth, from the vomica of a phthisic lung (No. 25) in nine successive breedings for 
six months; sixth, from a perlsucht knot (No. 39) in eleven successive breedings 
for nine months—were subcutaneously inoculated, and, moreover, from every one 
of the culturen four animals were inoculated. The mice were put in twos into 
roomy glasses. Some animals died after a few da3*s, apparently in consequence 
of the influence of the imprisonment. All the others visibly grew ill, the inguinal 
glands began to swell, the animals became emaciated and suffered from difficulty 
of breathing. In the course of four to six weeks they all died. The examination 
