AETIOLOGY OF TUBERCULOSIS. 
167 
used up to tliis time were the following : Inoculatiou into the anterior eye-cham¬ 
ber of rabbits, injection into the abdominal cavity, injection into one of the 
larger veins, inhalation of reincultur of tuberculous bacilli. 
Inoculation of Reinoulturen in the Anterior Eye-Chamber. 
A cut several millimeters long was made in the cornea, and, moreover, on 
the upper border of the same, and by means of a blunt hook as small a crumb as 
possible of a reincultur was pushed through this into the anterior eye-chamber 
of a rabbit. Some practice and patience are required for this, and on this ac¬ 
count I afterward followed another method. The cultur, rubbed to pieces in dis¬ 
tilled water, was taken into a syringe, whose needle must be very fine and sharp. 
The point can easily be pricked through the cornea into the anterior chamber, 
and the liquid can then be injected into it. This last method is so far more fav¬ 
orable as that the quantity of the infectious material can be very easily con¬ 
trolled. One sees plainly, in moving the piston of the syringe, how the cloudy 
injecting fluid mingles with the aqueous humor in the eye chamber, and one can 
inject much or little liquid as he will. A minimum of bacilli can be brought into 
the anterior chamber, if the needle of the filled syringe be put into it, and with¬ 
out a real injection be taken out again, since traces of the liquid in the needle 
mix with the water of the chamber, even if the piston of the syringe be not set in 
motion. 
Twelfth experiment: Little crumbs of a reincultur from a caseous-pneumonic 
lung (No. 27) cultivated for three months in five successive breedings, were put 
into the anterior eye-chamber of three rabbits. After a few days an intense iritis 
developed, the cornea soon became cloudy and yellowish gray. The animals then 
became emaciated very rapidly. They were killed after twenty-five days, and 
beside the caseous-purulent destruction of the bulbus, swelling and caseous de¬ 
generation of the lymph-glands of the lower jaw and of the base of the ear, very 
numerous tuberculous knots, partly with whitish centres, were found in the lungs. 
Thirteenth experiment: Reincultur from a perlsucht lung (No. 19) culti¬ 
vated for three months in five successive breedings, was rubbed with sterilized 
blood serum, and injected into the anterior eye-chamber of two rabbits. A third 
rabbit received just such an injection of pure blood serum. In the case of the 
first rabbits the same appearances as in the twelfth experiment occurred. Iritis 
quickly running its course, and cloudiness of the cornea in a few days. The 
eyes of the third rabbit showed no change. The animals were killed after 
twenty-eight days. The rabbit into whose eye the pure blood serum had been 
injected showed itself perfectly healthy; the other two had caseous bulbi, swollen 
lymph-glands provided with caseous spots on the lower jaw and beside the base 
of the ear, and numberless tuberculous knots in the lungs. 
Fourteenth experiment: Four rabbits concerned. Pure blood serum was 
iujected into the anterior eye-chamber of the first. The needle of the syringe, 
which contained blood serum with an addition of reincultur (from tuberculosis of 
monkey No. 12, cultivated four and one-half months in eight successive breedings) 
was put into the anterior eye-chamber of the second, but the piston was not moved; 
several drops of blood serum mixed with reincultur were injected into the anterior 
eye-chamber of the third and fourth rabbits. In the case of these last two animals 
