198 
R. KOCH. 
(miliary tubercles of man, cultivated for five months in eight successive breedings;) 
the third from reincultur No. 1 (lung phthisis of man, cultivated for seven months 
in twelve successive breeds) ; the fourth from reincultur No. 16 (perlsucht knot, 
cultivated for five and a half months in nine successive breedings) ; the fifth from 
reincultur No. 13 (tuberculosis of monkey cultivated for three months in five 
successive breedings). Just the same was done with five guinea pigs. Of the 
last, one each died on the twelfth, fourteenth, fifteenth and twenty-first days. 
The controlthier was killed on the twenty-second day. Of the cats the fourth 
died on the twenty-second day, the third on the twenty-seventh day, the other 
animals were killed on the twenty-eighth day. All the animals into which the 
bacilli liquid had been injected showed the familiar tuberculous changes in a 
state of development corresponding with the space of time since the injection. 
As well the cat as the guinea pig into whose abdominal cavities pure blood serum 
had been injected, were wholly free of tuberculosis. This experiment, like sev¬ 
eral previous ones, was undertaken to test any possible differences which might 
exist in the results of bacilli culturen originating in the various forms of tuber¬ 
culosis. But this time also the expectation cherished was not fulfilled ; for the 
tuberculosis generated by the various reinculturen conducted itself exactly the same 
in all, in the cats as well as in the guinea pigs. 
Twenty-first experiment: Reincultur from tuberculosis of monkey (No. 
11, cultivated for five months in ten successive breedings) was injected into the 
abdominal cavity of five rats. These animals were fed for some time beforehand 
with the dead bodies of tuberculous guinea pigs. In the case of other rats which 
belonged to the same feeding experiment and had been killed, only individual 
grey knots had been found a few times. But when the rats, into whose abdom¬ 
inal cavity tuberculous bacilli had been injected, were killed, after five weeks, 
numberless tuberculous knots were found in the lungs, in the greatly enlarged 
spleen, and in the liver and omentum. 
Twenty-second experiment: Reincultur (No. 24, from a phthisic lung, cul¬ 
tivated for five months in nine successive breedings), rubbed up in distilled water, 
was injected into the abdominal cavity of the following animals ; six guinea pigs, 
three cats, four white mice, four domestic fowls, eight doves. The guinea pigs 
died in from ten to seventeen days, the cats one each on the fifteenth, twenty- 
third and twenty-fourth days. The results as found in the dissection were the 
same as in the other experiments. The mice, fowls and doves, to be sure, re¬ 
mained alive, but were rough, thin, and seemed sick. As they did not recover, 
they were all killed at the end of ten weeks. The mice showed the same appear¬ 
ance as the white rats; they had quite numerous tuberculous knots in the lungs 
and very many in the greatly enlarged spleen. In the fowls and doves were 
found such knots as have already been described in the intestines and in the 
liver. 
Injection of Reinculturen in the Veins. 
By this method the infection of the animal is wrought most quickly and in 
the manner most productive of result. The body is at once overflowed by means 
of the blood with as great a quantity of the infectious matter as one wishes. 
Said matter has no need to overcome the hindrances put in its way, by the lymph- 
