/ETIOLOGY OF TCBEUCULOSrS. 
199 
glands, etc., as when other methods are used, but spreads itself immediately over 
all the organs aud causes a great and quite uniformly distributed tuberculous 
eruption. The mode of infection has plainly the greatest resemblance to that of 
miliary tuberculosis in man, where the tuberculous virus also makes its way into 
the blood and so is carried everywhere. By the help of injection into the veins 
tuberculous knots can be called forth in all the organs in such short time, and in 
so enormous numbers as is never the case in spontaneous tuberculosis; a mistak¬ 
ing of one for the other is therefore here completely excluded. The liquid in 
which the reinculturen of tuberculous bacilli were divided as finely as possible 
was filtered through fine gauze, in order to keep back all coarser particles, and 
then injected with one of the formerly described disinfected syringes, into the 
vena jugularis, or according to Aufrecht’s example, direct into the ear-vein of a 
rabbit which had been laid bare. 
Twenty-third experiment: Of twelve rabbits, two had a half cubic centi¬ 
meter of pure blood serum injected into the ear-vein; four rabbits received in 
the same manner blood serum with an addition of reincultur No. 11 (tuberculosis 
of monkey, cultivated for six months in eleven successive breedings—compare the 
seventeenth experiment) ; three rabbits blood serum with reincultur No. 1 (from 
phthisic lung cultivated for six months in ten successive breedings); three rab¬ 
bits, blood serum with reincultur No. 19 (perlsuclit lung, cultivated four months 
in seven successive breedings). In the first days after the operation nothing 
striking was to be noticed in any of these rabbits. The two first remained brisk 
and vigorous, all the others began to breathe hard in the second week, and became 
emaciated with unusual rapidity. The first rabbit (injection with cultur No. 1) 
died after eighteen days; the second and third (injection with cultur No. 11) after 
nineteen days ; the fourth (cultur No. 19) after twenty-one days ; the fifth (cul¬ 
tur No. 1) after twenty-five days; the sixth and seventh (cultur No. 11) after 
twenty-six and twenty-seven days ; two other animals on the thirtieth and thirty- 
first days. The last and the two controlthiere were killed on the thirty-eighth day 
after the injection. In the conduct of the lungs and other organs of the animals 
treated with the various culturen, as in former similar experiments, no distinction 
could be observed. In all the animals numberless miliary tubercles were found 
in the lungs. The liver and spleen of all these animals contained an extraordi¬ 
nary number of tubercles. In those which died first the knots were smallest, but 
also most numerous. It was plain that the great number of tubercles had caused 
such an early death. In the animals dying later the number of knots was some¬ 
what smaller, but their size, on the contrary, decidedly larger. The two control¬ 
thiere were found on dissection without a deposit of tubercles in any organ. 
Twenty-fourth experiment: Pure culture of lupus, No. 35 (cultivated for 
five months in eight successive breedings), rubbed up with distilled water and in¬ 
jected into the ear-veins of five rabbits. These died from the thirteenth to the 
eighteenth day after tike injection and in the dissection showed the same state of 
things as in the rabbits of the former experiment. 
Twenty-fifth experiment: Pure culture of monkey tuberculosis No. 11 (cul¬ 
tivated for six months in twelve successive breedings), rubbed up with distilled 
water, was injected into the vena jugularis of ten rabbits which were intended 
for experiments in inhalation with means of hindering the development. They 
