EXPERIMENTAL PATHOLOGY. 
227 
the remarkable fact that the tumefaction of the testicles, in¬ 
stead of showing itself only after eight days, had become well 
marked as early as the second or the third day after the inocu¬ 
lation. On the eighth or tenth it has reached considerable 
proportions, and death has occurred earlier, generally in from 
twelve to fifteen, and sometimes in from four to eight days. 
The peritoneum and viscera of a guinea pig killed two 
days after having received a peritoneal inoculation of a small 
quantity of the culture of the bacillus of glanders, will gen¬ 
erally preserve a healthy appearance. But if the tumefied 
testicles are examined, lesions appear of a nature more ad¬ 
vanced and characteristic. Mr. Loefiler has pronounced these 
lesions to be a glanderous orchitis, or epididymitis, but this 
is an error, the lesions always beginning in the vaginal sac. 
From the second day following the intra-peritoneal inocula¬ 
tions, both layers of the vaginal serous tissue are literally 
covered with a mass of white-yellowish granulations, of the 
size of a pinhead, and on the third or fourth day these layers 
become intimately united by a thick, purulent exudate, and 
rich in bacilli, the scrotum at the same time becoming adher¬ 
ent, inflamed and red. 
If the tumor is divided, the testicle proper, as well as the 
epididymi, are seen to be free from lesions, which do not ex¬ 
tend beyond the tunica albuginea, the testicular substance 
being at the same time perfectly healthy. 
Out of forty post mortem examinations of animals killed 
by sub-cutaneous or intra-peritoneal inoculations, only two 
presented testicular lesions. It is not then, as has been be¬ 
lieved, an orchitis or epididymitis of a glanderous nature; it 
is only the testicular envelopes, the vaginal sac, and then the 
scrotum, which are primitively and exclusively affected. This 
exclusive Localization in the serous sac of the testicle is ob¬ 
served in both forms of inoculation, but in the peritoneal it 
takes place much sooner , having been observed even as early 
as the second day. 
This rapid and characteristic localization of the diseased 
process of glanders upon the testicular envelopes in the gui¬ 
nea pig, may then be utilized in ascertaining the glanderous. 
