272 
FRANK S. BILLINGS. 
ders into the circulation by injecting the same into the saphe¬ 
na vein. When a considerable quantity was injected (i to 2 
centimeters) the animal presented the phenomena, of intense 
fever and great constitutional disturbances at the end of seven 
days ; the skin was marked by numerous nodules in its sub¬ 
stance, which eventually ulcerated, yielding a sero-sanguino- 
lent secretion conformable to that generally seen in these 
lesions. Death resulted in from three to six days. Macroscop- 
ical examination revealed the presence of fine glanders gran¬ 
ulations in the liver and spleen, but more rarely and less 
extensively in the lungs. Cover glass preparations of the 
cutaneous secretion and blood of the heart revealed the pres¬ 
ence of glanders bacilli. These experiments with large quan¬ 
tities of pure bouillon cultures demonstrated the fact that a 
fatal form of the disease could be produced thereby in the 
d °g- 
If, on the contrary, one injects into the same vein a smaller 
quantity of the same culture, the results are of a much less 
grave character, the cutaneous eruption is not so acute or se¬ 
vere and the animal recovers. This is a new and striking 
example of the fact shown by Chauveau of the proportional¬ 
ity which exists in certain diseases between the dose of the 
virus and the results following on the same. 
With dogs thus treated and on the lapse of some weeks 
or months after recovery, extremely strong doses of a very 
virulent culture were again injected into the vein in question, 
doses that would have been invariably fatal in an animal (dog) 
not so prepared. Neither local nor general phenomena were 
observed to follow in most of these experiments, though a 
mild febrile condition and a slight cutaneous eruption was 
occasionally seen. These experiments demonstrate that arti¬ 
ficial immunity can be induced iu dogs in the manner in¬ 
dicated.” 
So much for our author. 
Certainly we have here strongly conclusive experimental 
testimony in favor of my hypothesis. The next question is 
to decide whether by carrying the first induced cutaneous 
affection .to healthy dogs in serial succession we can induce a 
