EXTRACTS FROM GERMAN PERIODICALS. 
35 
butter—also evidencing the mould fungus—mucor mucedo, 
and another immobile bacteria. 
A series of later experiments developed the fact that a 
winter’s cold of i6° F. for fourteen days and the addition of a 
13 per cent, sodium chloride solution, were unable to influence 
the germinating colonies.— Allgem. Med. Central Zeits. 
CULTURES OF ACTINOMYCOSIS. 
M. Wolf and Dr. J. Israel were successful in obtaining an 
artificial colony of this micro-organism, and in transplanting 
the same upon animals. 
The cultures grow well upon agar, under the usual pre¬ 
cautions, and manifest a decided disposition to nodule forma¬ 
tion ; the latter appearing on the third or fifth day. 
The collections flourish best in a temperature of 95 0 to 
98° F. the growth being independent of the presence of 
oxygen—being, therefore, an anasrobic organism. 
The actinomyces fungus under the microscope is of var¬ 
ious size and form ; short and long single lengths and articu¬ 
lated or compound threads being in the field of vision; there 
may also be detected a spiral formation and numerous cocci. 
The characteristic and notable form of this fungus, to wit, 
that of an Indian club, does not appear in the cultures. 
The number of experiments upon animals amounted, in 
all, to twenty-two; 18 rabbits, 3 guinea pigs and one sheep. 
The two former species gave positive results, but the inocula¬ 
tion upon the sheep failed. 
Upon post mortem the infected animals exhibited the 
characteristic new formation which in turn yielded the typi¬ 
cal club-shaped bodies, single and radiating. These, when 
placed upon various soils gave the results above mentioned, 
losing their shape incidentally. 
Previous to this success of Wolf and Israel, it was not pos¬ 
sible to cultivate the actinomyces fungus. — Virchows Archiv. 
