124 
R. ARTMANN. 
\ 
o’clock. Two of these animals died at noon of the day fol¬ 
lowing, or in from twenty-eight to thirty hours. The third 
was found dead in his cage the next morning. The autopsi- 
cal demonstrations revealed tumor lienis and catarrhus intesti- 
nalis hemorrhagicus , and in all three cases the microscope con¬ 
firmed the anthrax condition. 
From these lesions I made cultures and impregnated some 
of the silk threads, and treated them by soaking them in a 
sarena solution of 1-2000, and even stronger, the result of the 
inoculation being negative as to the development of anthrax, 
and only in a few exceptional cases being followed by a little 
suppuration at the point of insertion. 
Experiment I.—Operating with a solution 1-2500, I satu¬ 
rated ten silk threads, and used them as follows : 
No. 1 was used immediately after being dipped in the solution. 
No. 
2 
after 
remaining 
1 
a minute 
No. 
3 
<< 
u 
I 
minute. 
No. 
4 
u 
u 
I 
1 << 
2 
No. 
5 
u 
u 
3 
<< 
No. 
6 
u 
a 
5 
u 
No. 
7 
u 
a 
20 
u 
No. 
8 
u 
u 
40 
(( 
No. 
9 
u 
u 
60 
u 
No. 
10 
u 
u 
2 
hours. 
Ten guinea-pigs were thus inoculated on the 31st of March, 
at 4 p.m. The animals were feeling very well until the after¬ 
noon of the 4th of April, when my assistant reported No. 2 
at being sick. When I saw him I observed that not only 
No. 2, but also No. 1 and 4 were affected, though less so than 
No. 2. At noon of the day following the inoculation the ani¬ 
mals had all eaten well. 
The symptoms then observed were the following : slightly 
comatose ; no appetite; great thirst; sometimes very ner¬ 
vous, or again lying down to jump up again, as in convul¬ 
sions. These symptoms subsided on the 5th of April. The 
other animals showed no signs of illness. 
On the 6th of April, at 8.20, I inoculated six more guinea- 
pigs, as I had done before, as a means of control. On the 
