126 
R. ARTMANN. 
bouillon, a most interesting fact was observed, viz., that after 
twelve hours in the breeding apparatus the bacilli, though 
they had grown, did not present the characteristic gran¬ 
ulated appearance—a fact which was confirmed by the cul¬ 
tures made on glass plates and reagent glasses. The cultures 
grew very slowly, and were not of their usual size or aspect. 
After thirty-six hours I could count only about twenty colo¬ 
nies on ten glass plates. 
Inoculations were made in five cases with this material, 
but in one only were there signs of sickness after one hun¬ 
dred and thirty hours, and in this I was unable to determine 
positively that his sickness was of the nature of anthrax. 
From this I infer that with a solution of 1-5000 the bacillus 
loses its aspect and its growing properties, but that it may 
still be effective in a first inoculation. 
Experiment V. — Sarena solution, 1-7500; day of vac¬ 
cination, 31st of March at 5.30 P.M. On the night of April 3d 
Nos. 1, 2, 3, 4 and 6 were dead, and all the others very sick, 
especially No. 5, which was in a very comatose condition. 
They recovered after from twelve to eighteen hours of sick¬ 
ness. Both the post-mortem and the microscope showed an¬ 
thrax lesions. Cultures grew up in twenty-eight hours, and 
inoculation proved fatal in two out of ten cases. 
Experiment VI.—Sarena solution 1-10000; day of inoc¬ 
ulation, 31st of March at 6 p.m. Nos. 1 and 7 died on the 
second day ; No. 8 on the third, and Nos. 9 and 10 recov¬ 
ered in four days after severe sickness. Cultures were also 
prolific but slow. 
Conclusions. —The results of thes■' experiments are that 
a solution of 1 in 2000 is a good and sure prophylactic and 
preventative. It immediately kills all germs. Experiments 
relating to the direct contact of sarena with other bacilli-cul- 
tures are not yet completed, but will, I believe, give still bet¬ 
ter results. I have also experimented with the bacillus tuber¬ 
culosis, and I am satisfied that a solution of 1 in 3,500 is per¬ 
fectly sufficient to arrest completely the further fructification 
of the bacillus. A solution of 1-3000 produces some effect, 
but fails to destroy the bacilli or germs. 
