VIRULENCE OF BLOOD AND MUSCLES IN TUBERCULOSIS. 231 
vein. The hair having been clipped closely from the middle 
part of the jugular furrow, the skin was washed with a strong 
solution of mercuric chloride in water. The fleam or lancet 
used to open the vein was sterilized in a 5 per cent, solution 
of carbolic acid in water, and the vessel in which the blood 
was caught had previously been sterilized, either by exposure 
to dry heat for one hour at 160° C., or by steaming for half 
an hour at ioo° C., and for a subsequent quarter of an hour 
at Ii5°-i2o 0 C. The blood was dehbrinated by whipping 
with a bunch of iron wires previously heated to redness in 
the flame of a Bunsen burner. The blood was injected into 
the peritoneum with an extemporized instrument on the 
principle of Koch’s syringe. This instrument consisted 
simply of a hypodermic needle attached to one end of a piece 
of glass tubing either by cement capable of resisting a dry 
temperature of 160° C. or by a short piece of rubber tubing 
embracing both needle and glass tube at their point of con¬ 
tact. In the latter case the instrument was sterilized by 
steaming in an autoclave for half an hour at ioo° C: and then 
for a quarter of an hour at 120° C. In inoculating the ex¬ 
pelling force was obtained by attaching the ball of a “ hand 
spray apparatus ” to the free end of the glass tube. 
Experiment I.—The blood in this case was taken from a 
cow with enormous tuberculous tumors (lymphatic glands) 
in the parotideal region and in front of the shoulder. Each 
of three full-grown rabbits had 6.8 cc. of the dehbrinated 
blood injected into the peritoneum. 1 All three rabbits were 
killed 41 days after inoculation. The peritoneum and all the 
organs of the qody appeared to be perfectly normal, save 
1 I may here remark that as long as the material used is bland and unirrita¬ 
ting, such as blood, milk, or meat juice, intra-peritoneal inoculation is a simple 
and apparantly almost painless operation. In the earlier experiments, in which 
the blood was injected cold, the animals for a few minutes after the operation 
lay prone on the abdomen with the hind legs extended, but since I have adopted 
the method of raising the liquid to be injected to the body temperature, the 
animals have in general borne the operation without any sign of discomfort. 
When the stomach and intestines are not overloaded with food materials 5 cc. or 
more may be injected into the peritoneum of a full-grown guinea-pig and twice 
that amount into a rabbit. 
