234 
j. m’fadyean. 
Experiment IV.—This experiment was similar to the 
preceding one, but the blood was from a different horse and 
withdrawn 20 hours after the intra-venous inoculation. One 
of the guinea-pigs received 4 cc. and the other 2 cc. of the 
defibrinated blood. 
Both guinea-pigs were killed 23 days after inoculation, and 
the post-mortem showed in neither case the least trace of 
tuberculosis. 
Experiment V.—In this instance the blood was withdrawn 
(from a third horse) 48 hours after the intra-venous inocula¬ 
tion, and after defibrination 5 cc. of it were injected into the 
peritoneum of each of two guinea-pigs. 
Both guinea-pigs were killed 34 days after inoculation, 
and both were found to be absolutely healthy. 
2 . Experiments with Meat Juice. 
The following three experiments were made with the 
juice expressed from the muscles of the three horses referred 
to in connection with the foregoing series. And here again, 
although the results were negative, or rather because they 
were negative, it appears desirable to state that at every step 
strict precautions were taken to exclude the risk of accidental 
infection of the animals under experiment. The superficial 
dissection required to expose the muscles was made with a 
sterilized knife, and a second sterilized knife was used to cut 
out the portions intended to furnish the juice for inoculation. 
In each case the muscular tissue was taken from the pectoral 
region, and the juice was expressed from it by means of a 
sterilized meat-press, and received in sterilized vessels. The 
syringe used for inoculation was of the extemporized pattern 
previously described, and in each case it was carefully steril¬ 
ized before use. 
Experiment I.—Two guinea-pigs were inoculated intra- 
peritoneally with muscle juice, one receiving 4 cc. and the 
other 3 cc. The juice was obtained from the muscular tissue 
of a horse killed 10 day after 5 cc. of a liquid enormously 
rich in tubercle bacilli had been injected into the jugular vein. 
Both guinea-pigs were killed 26 days after inoculation, and 
found to be perfectly healthy.,) 
