294 
Y. A. MOORE AND F. L. KILBORNE. 
Very marked indol and phenol reactions were obtained 
with a culture that had grown for eighteen days. Its ther¬ 
mal death point, power to resist drying, and to survive in 
sterilized water were determined. An exposure in a water 
bath for hve minutes at a temperature of 58° C. destroyed its 
vitality. Upjn cover-glasses exposed to the atmosphere 
under a bell jar it lost its power of development when inocu¬ 
lated into bouillon in forty-eight hours. It perished in ster¬ 
ilized distilled water in eleven days. • 
It will be observed that morphologically and biologically 
this bacillus does not differ appreciably from the swine-plague 
germ, except, perhaps, in its indol reaction, which I have 
never found in similar cultures of swine-plague bacteria. It 
was found by Dr. Smith in one case. 
Pathogenesis. — Mice, pigeons and guinea-pigs remained 
well after an inoculation with from o 1-0.2 cc. of a fresh bouil¬ 
lon culture. Rabbits inoculated subcutaneously with a simi¬ 
lar quantity of the virus developed quite severe local ab¬ 
scesses but finally recovered. A rabbit inoculated intraven¬ 
ously with 0.4 cc. of a fresh (twenty-four hour) bouillon 
culture perished on the fifth day with exudative pleuritis, 
pericarditis and peritonitis. A second rabbit inoculated with 
a similar quantity of the culture died on the sixth day. It 
exhibited a cellular exudate over both parietal and pulmon¬ 
ary pleura. The dorso-median portion of the principal lobes 
of both lungs were in a state of red hepatization, the cephalic 
lobes were collapsed ; heart muscle was pale, and contained 
dark blood clots; mucosa of trachea injected; liver pale, 
fatty ; spleen enlarged, dark and friable ; kidneys hypersemic ; 
in the intermuscular tissue about left knee and right elbow 
joints were small abscesses containing pus and rabbit septi¬ 
caemia bacteria. Stained cover-glass preparations from the 
various organs contained very few bacteria. Those from the 
exudate and pus from abscesses exhibited an innumerable 
number of polar stained germs. 
A short series of inoculations were made for the purpose 
of accelerating the virulence of this bacillus. The results 
were negative. 
