A TUBERCULOUS HERD. 
647 
evening of March 7th by myself, assisted by Drs. Cooper 
Curtice and R. A. McLean, Inspector of Cattle for the New 
York State Board of Health, who also aided in conducting the 
autopsies and all the other work incident to the experiment. 
The tuberculin was prepared by mixing one part of this 
agent with nine parts .of a 5 to 1000 solution of carbolic acid in 
sterilized distilled water, and injecting a suitable quantity under 
the skin in the scapular region (the space anterior to the spine 
of the scapular) by means of a clean hypodermic syringe. 
For an average sized cow 2]/ 2 cc. of this mixture was used, but 
for large cows 3 cc. and for one very large cow 3 x / 2 cc. were 
injected, the younger animals receiving proportionately smaller 
doses. The hair was first clipped close over a space about the 
size of a silver dollar on the right shoulder where the injection 
was to be made, and the temperatures of those who were to be 
experimented upon were taken before commencing the inocula¬ 
tions. All the adult animals, the heifer that had aborted, and 
three of the yearlings were used for the test. 
The cattle were driven to the Tenderers on the afternoon of 
March 8th, a few post-mortems were made March 9th, in the 
afternoon, upon those that died in the night, or weak ones that 
had to be killed because the stronger ones were knocking them 
around, the remainder and greater number of autopsies were 
made March 10th. The reason that so little was done March 
9th was that it was the breaking up of Winter, a warm rain 
turning two feet of snow into slush, and making the country 
roads almost impassable. 
The temperatures of the animals were taken first at 8 o’clock 
on the evening of March 7th, and this occupied an hour and a 
half; the inoculations commenced at 9:45 and were completed 
at 10:30; and the first temperature after the inoculations was 
taken at 1 A. M. March 8th, and it took about an hour to go 
through the lot. 
Below is a separate table for each animal, giving the suc¬ 
ceeding temperature and port-mortem notes. 
Tag No. 19.—High grade Jersey cow, seven years old, some- 
