14 
E. ZSCHOKKE. 
recovered without treatment and was returned. On the 14th 
of November this animal, whose milk yield had reached four 
liters, again showed infectious mammitis in one of the quarters 
which had recovered spontaneously before. Such cases of 
spontaneous recovery with temporary relapse occasionally occur, 
and serve as proof of a certain immunity. 
Now for the attempts to cure. Earlier experiments have 
shown that it is impracticable to attempt to kill the streptococci 
or to obtain improvement by the injection of disinfectants in 
the udder on account of the great sensitiveness of the glands. 
Therefore, in these experiments only two substances, both 
harmless to the udder, were used, namely, potassium iodide, 
1:1000, in lukewarm water, and itrol, 1:4000, at about 
38° C. 
In each case the udder was milked out, and from one hun¬ 
dred to three hundred grams of the solution were repeatedly 
injected into the teat, being well distributed through the gland 
by massage. Care was taken throughout to prevent the spread 
of che infection. 
The animals showed neither indications of pain nor change 
in the glands, but the desired result did not follow. The secre¬ 
tion of the udder remained unchanged and the streptococci were 
found in nearly the same numbers as before. 
Experiments with antistreptotoxin from the Pasteur Insti¬ 
tute in Paris, with which some work had been done three years 
ago, were again resumed. This remedy was supposed to coun¬ 
teract the poison elaborated by the streptococci, and it was 
hoped that favorable results would follow its use in large doses 
or its injection directly into the udder. 
After injecting 200 grams of this serum subcutaneously, 
within a period of three days, without any such result, on the 
26th, 27th, 28th and 29th of July I injected about 30 grams of 
the remedy into a diseased milk cistern and distributed it well 
in the udder by rubbing and kneading. 
A noteworthy curative result was lacking. Cultures 
and microscopic tests added proof that the antistrepto- 
