BOVINE TUBERCULOSIS. 
319 
First lot consisted of animals i, 2, 3 and 4 and were sub¬ 
mitted to the gastro-intestinal tubercular infection. I11 order 
to accomplish this 475 grams of pleural tubercles, cut fine and 
mixed with bran, were given each subject. 
No. 3 was the only one that ate the whole of the mixture ; 
No. 2 ate about 100 grams mixed with three (3) liters of 
water. 
Cows Nos. 1, 2 and 4 having refused to eat the tubercular 
food, they were each drenched, May 14th, with 250 grams of 
tuberculous material taken from a mediastinal gland. The 
emulsion was made with lukewarm water. 
The infecting material for the other subjects consisted of an 
emulsion of a growth of Koch’s bacilli, taken from the milk of 
a tubercular cow, the emulsion being twice filtered through fine 
cambric. 
Four guinea-pigs were inoculated under the skin of the thigh 
with one drop of this emulsion, causing them to die between the 
29th and 42d day after the inoculation. 
The second lot consisted of Nos. 5 and 6, and they were 
made to inhale tubercular dust. This operation was performed 
by Mr. Nocard and his laboratory clerk. To accomplish this 
a cylinder of ducking two metres long, kept open by metal 
hoops and closed at one end, was used. A strap passed around 
the neck held the bag in place as high as the eyes, and a 
circular strap held the bag tightly against the face, permitting 
the animal only to breathe the air contained in the bag. 
A Collin insufflator was fixed into the small end of the ap¬ 
paratus and by this means 3 cubic centimetres of impalpable 
tubercular dust, obtained by the dessication at 37 0 of the emul¬ 
sion above referred to, then pulverizing and sifting the dessi- 
cated matters, it was introduced into the air contained in the 
bag. 
The operation lasted five minutes for cow No. 5 and four 
and one-half minutes for cow No. 6. 
The third lot consisted of cows No. 7 and 8. The experi¬ 
ment on cow No. 7 was made with the aid of the cloth bag 
