320 
MM. NOCARD AND ROSSIGNOL. 
above described, and a large Guasco atomizer. In six (6) min¬ 
utes ioo cubic centimetres of a saturated tubercular emulsion 
was atomized. 
For cow No. 8 a common atomizer had to be used, the other 
having got out of order, and the infecting tubercular emulsion 
had to be injected in the immediate vicinity of the nostrils, and 
in five minutes ioo cubic centimetres was so atomized. 
The fourth lot consisted of cow No. 9, and an intraveinal 
injection of 10 cc. of the tubercular emulsion was made. 
Fifth lot were milk cows Nos. 10 and n. They were cast 
on a straw bed, a fine round-ended tube was passed through the 
teat, so as not to wound the gland or the mucous membrane, and 
5 cc. of tubercular emulsion was injected in each of the left an¬ 
terior and right posterior mammae. 
Sixth lot was cow No. 12, about to calve. 10 cc. of tuber¬ 
cular emulsion was injected into her trachea. 
It was agreed that her calf, if born alive, would receive 
every day not only its mother’s milk, but also that of the two 
cows, Nos. 10 and 11, already submitted to the intra-mammary 
injection. 
It was agreed that the twelve cows submitted to the experi¬ 
ment shall every six days be subjected to the tuberculin test. 
Each lot will be considered in succession from the begin¬ 
ning of the experiment to the killing of the animals. 
FIRST TOT—GASTROINTESTINAL INFECTION. 
This was composed of cows Nos. 1, 2, 3, and 4, and they re¬ 
sisted the tubercular infection for a very long time, and at the 
time of their death they were apparently in the best of health. 
On the 32d day, that is the 9th of June, there was observed 
a slight reaction in consequence of the tuberculin injection, the 
8th, with cows No. 3 and 4. 
No. 3.—6 a. m., 39.5 ; 9 a. m., 39.5 ; noon, 38.6 ; 3 p. m., 
39 * 1 * 
No. 4.—6 a. m., 39.6; 9 a. m., 38.9 ; noon, 38.2 ; 3 p. m. 
39 - 3 - 
