646 
W. H. DALRYMPLE 
doubt, just stated, and, it is reasonable to presume that a great 
deal of the infection is washed from of? the surface of the 
ground, and of the vegetation, and carried away by running 
water, as streams, rivers, etc. This, of course, creates a menace 
to territory below, and through which such water passes. As 
an instance of this, we got infection on the pasture of our State 
Experiment Station, through the discharges of a charbon victim, 
belonging to a neighbor immediately above us, being washed 
into a branch which runs through it. And, the lands of our 
State, bordering on the Mississippi River, might easily be in¬ 
fected from the State of Mississippi to the north of us, as I under¬ 
stand numbers of the victims of the recent terrible epizootic 
there, before the authorities took action in the matter, were 
thrown into the river to float down. 
The third mode of infection is by way of the lungs or respi¬ 
ratory tract ; but, although the human subject contracts anthrax 
as “ wool-sorters’ disease,” by inhaling the desiccated spores 
from the wool of sheep that has been soiled with infected blood, 
I do not think that animals often receive infection in this way, 
and if so, it is of somewhat rare occurrence. At all events, I 
should consider this mode quite infrequent as compared to the 
others mentioned. 
PREVENTIVE INOCULATIONS 
were first made by Toussaint, but were apparently unsuccessful 
in obtaining the desired results. Pasteur, however, demonstrat¬ 
ing that immunity was produced by weakened virulence on the 
part of the organism, obtained an attenuated virus by cultivat¬ 
ing the bacterium at a temperature of 42 to 43 degs. C. in the 
presence of oxygen. There are other processes of preparation of 
the virus, but the lymph that I am most familiar with, and the 
one which has been put into practice in almost all Continental 
European countries, is that prepared by the Pasteur method. 
The dose of this virus comprises two inoculations. The first 
lymph, or first half of the dose, is that which has been culti¬ 
vated, under the conditions above mentioned, for about 24 days; 
the second lymph, for about 12 days. The first dose is, there- 
