HUMAN TUBERCULOSIS IS TRANSMISSIBLE TO CATTLE. 
737 
He inoculated a calf into the eye and into the subcutaneous tis¬ 
sue with human bacilli, which had showed to be verv virulent 
in the guinea-pig and in the rabbit. 
On the first day the eye showed a severe irritation, which 
disappeared at that point until when, 15 days later, no inflam¬ 
matory trouble remained. After 8 days, the cornea had become 
transparent and one could distinguish the iris, on which had 
formed a small yellowish deposit, which disappeared little by 
little. O11 the flank a small swelling had formed, which dis¬ 
appeared without leaving a trace. About 3 months after the 
inoculation the calf was slaughtered and at the autopsy no 
tubercular lesion could be found whatever. The inoculated 
rabbits and guinea-pigs succumbed to a generalized tuberculo¬ 
sis. Another calf infected in the same manner with the bovine 
bacillus, of which the virulence had been increased by passing it 
through a rabbit, was taken by a generalized tuberculosis. 
Own experiments. The startling results published in 1898, 
by Theobald Smith, caused me to experiment on this line, in 
order to contribute, if possible, to the elucidation of a question 
which, at first, seemed to attract but very little the attention of 
bacteriologists, although it is from a hygienic point of view of 
the utmost practical importance, as we have just seen at this 
very congress. 
In the month of March, 1899, two calves, one 8 weeks and 
the other 4 weeks old, served for a first experiment. 
They were first subjected to the tuberculine test. After an 
injection of 10 centigrammes of tuberculine, no reaction oc¬ 
curred, so that the animals could be considered as being free 
from tuberculosis. The milk on which they were fed came 
from a tuberculine tested cow, which did not react. 
I. The 8 weeks old calf received into the abdominal cavity 
a culture on glycerinized potato, obtained from the exudate of 
a child affected with a tuberculous meningitis, the culture be¬ 
ing procured from the laboratory of Prof. Spronck, of Utrecht. 
Its virulence had been tested on the rabbit, and on the guinea- 
Pig. 
