740 
M. H. J. P. THOMASSEN. 
microscopic examination proved the tuberculous character by 
their structure, and by the presence of the specific bacillus. 
No tubercle showed signs of calcification. The results of the 
post-mortem examination shows that we have to deal with a 
fairly well generalized tuberculosis. The route followed by the 
bacilli from the eye to the lungs was evident. 
III. In the month of December, 1899, a heifer of about 2 
years old, and being in the best of condition, served for another 
experiment. In November, the animal, after an injection of 
0.25 G. tuberculine, showed only a reaction of 0.5 0 C. On the 
1st of December a rich culture on potato was injected into the 
trachea of the animal. It had been grown from a tuberculous 
kidney of a man. No trouble could be observed as a conse¬ 
quence, and on the 13th of February, 1900, an injection of 0.30 
G. tuberculine, caused a thermic reaction not exceeding i° C. 
O11 February 16 an entire culture was injected into the 
thorax and another into the abdominal cavity. Of the latter, a 
small quantity was spilled into the muscles of the left flank. 
On the next day, the temperature ran up to 40.3 ; the ani¬ 
mal was dull and showed little appetite. After two days, its 
condition had again become normal. Bit by bit a hard tumor, 
of the size of a fist and easily movable, had formed in the flank 
at the point of inoculation. After three weeks this, was opened, 
and after cutting through a fibrous envelope of the thickness of 
a finger, a yellowish purulent material escaped. It was rich in 
specific bacilli, which we were able to cultivate. Those cultures 
were characterized by a pale color. The wound, which was 
not treated with antiseptics, cicatrized .little by little, so that 
soon there remained nothing but a slight fibrous thickening. 
In the month of July, 1900, the tuberculine test was repeated 
without producing a notable reaction. The heifer was slaugh¬ 
tered in April, 1901. The animal, splendidly fat, showed not 
the least tuberculous lesion. 
IV. A fourth heifer, about 2 years old, was also inoculated, 
after a preceding tuberculine test, into the anterior chamber of 
the right eye with bacilli grown from the kidney of a man. On 
