|j aodeiate numbers. At this time, however, no espeeial difference 
oulcl be detected related to a difference of situation, while in torpid 
pecimens the bacteria were apparently no more abundant than in 
I hose more active. On the 27th of the month, four bugs were 
xamined from Conner’s field, and two from one of the others, but 
.0 bacteria whatever were found in any of them. These bugs were 
aken from the husks of the corn. They were in a sluggish condi¬ 
gn, and apparently had ceased to feed. 
Believing that I had now obtained as definite proof as was possi- 
le by this method that the bacteria observed were the cause of 
be remarkable diminution of the bugs in one of these fields, I next 
ttempted the artificial cultivation of the microphytes, with a view 
d getting them free from mixture with other substances, for more 
areful and convenient study. 
I also wished to see whether the exposure of healthy chinch-bugs 
) fluids containing the bacteria would have any effect upon the 
isects; and, if it would, whether those so affected would themselves 
Dnvey the contagion to others. 
A number of culture tubes were made, similar in character to 
lose ordinarily used for the pure cultivation of microphytes, and 
lied with hot infusions of corn and beef, made by boiling the pith 
P cornstalks and small pieces of beef in a test-tube for fifteen or 
jventy minutes. Some of these were carefully infected in the usual 
fanner with fluids from crushed chincli-bugs, while others were left 
ee. That containing the corn infusion produced only Bacterium 
rmo and Bacillus subtilis. The bacteria from the insects developed 
aly in the infected tube containing the beef infusion. In this tube 
iyriads of these bacteria occurred, both in zoogloea masses and as 
rparate individuals, but no other micro-organisms appeared. In 
le of the fields which had been worst attacked by chinch-bugs, the 
aves were dead as high as the ears. Here it was noticed that the 
heath of the leaf was often gummed to the stalk by a thick exu- 
ition, like half-dried glue. A few dead adults were noticed here, 
rabedded in mold, but of a different kind from that seen at Jack¬ 
al ville. When portions of this exudation were moistened and 
udied under the microscope, the fluid was found thick with bac- 
ria, indistinguishable from those occurring in the bodies of the 
sects, and the same were thickly scattered through the translucent 
asses of viscid jelly. The inference was plain that they were de¬ 
wed from the excrement of the chinch-bug, in which they had 
mtinued to develop. 
