TETANUS TOXOID 
ALUMINUM PHOSPHATE ADSORBED 
For active immunization against tetanus 
DOSAGE: For primary immunization, two doses of 0.5 cc. 
each, injected intramuscularly at four to eight week inter¬ 
vals, followed by a third injection of 0.5 cc. one year later. 
Composition 
Tetanus Toxoid Aluminum Phosphate Adsorbed, Wyeth, 
is a refined toxoid. Refinement is accomplished by methods 
perfected through the research division of Wyeth Laboratories. 
These new methods of refinement eliminate at least 97 per cent 
of the nonspecific nitrogen, yet the antigen remains unchanged. 
Highly purified toxoids are desirable since they contain con¬ 
siderably less non-toxoid bacterial proteins and broth constitu¬ 
ents which are capable of producing allergic or side reactions 
in their own right. Also highly purified toxoids increase the 
efficiency of mineral adjuvants since both the impurity and 
toxoid proteins compete for adsorption on to the mineral carrier. 
Adsorption of this highly purified antigen on a definite pre¬ 
determined quantity of aluminum phosphate, a mineral 
adjuvant, prolongs and enhances the antigenic properties by 
retarding the rate of absorption. Aluminum phosphate has been 
shown to be a superior mineral carrier of antigen by various 
investigators both as regards immune response and incidence of 
local reaction to administration. 1 2 > 3 
The purity of tetanus toxoid is expressed as Lf (units of 
flocculation) per mg. of protein nitrogen. The new Wyeth 
methods of refinement of tetanus toxoid result in a purity no 
less than 1500 Lf per mg. of protein nitrogen. This compares 
with crude fluid toxoids (Ramon) which contain approximately 
20 to 60 Lf per mg. of protein nitrogen and alum precipitated 
toxoids which have a purity of approximately 250 Lf per 
mg. nitrogen. 
Indications 
"Everyone should be actively immunized against tetanus. ”4 
This is especially true since approximately 50 per cent of 
