Nov. is, 1915 Gossypol, the Toxic Substance in Cottonseed Meal 263 
These products, differing in purity, have been designated by us as “gossy¬ 
pol extract,” “precipitated gossypol,” and “gossypol ‘acetate.'” All 
proved toxic to rabbits. 
The method of preparing the gossypol and other feeds is shown in the 
accompanying outline. 
OUTLINE or METHOD OF PREPARING FEEDS 
Whole cottonseed. 
Decorticated at mill. 
Hulls. 
Kernels, ground and sifted. 
“Fines” (100 per cent of 
kernels). 
Brownish oil (34 per cent of kernels), 
feed 290a. 
Extracted with petroleum 
solvents. 
Residue I (66 per cent of 
kernels), feed 290. 
Gossypol extract 
(2 per cent of kernels), 
feed 318. 
Precipitated with 
petroleum ether. 
Precipitated gossypol, 
feed 320. 
Treated with 
glacial acetic acid. 
Filtrate, 
feed 340. 
Recrystallized gossypol*- 
‘ ‘ acetate, * ’ 
feed 321. 
-Crystalline 
gossypol 
“acetate,” 
feed 319. 
Feeds 290, 318, 319, 320, and 321 are very toxic. 
Feed 316 is very slightly toxic after long feeding. 
Feeds 331 and 340 are nontoxic. 
Extracted with ethyl ether. 
Residue II (64 per cent of 
kernels), feed 316. 
Extracted with boiling 
alcohol. 
Extract, 
feed 330. 
Residue III, 
feed 331. 
