266 
Journal of Agricultural Research 
Vol. V, No. 7 
Two guinea pigs, A and B, were tried with this feed. Guinea pig A 
was off its feed at the time from eating precipitated gossypol spread on 
corn meal (feed 318). An attempt was made to give it kernels in which 
the gossypol was not so easily detected, but the animal would not touch 
them. 
Guinea pig B had eaten feed 316 for 50 days and had gained in weight. 
After it had been on corn meal and molasses (feed 317) for about a week, 
it was placed upon feed 290 (7 gm. of kernels with molasses). It ate only 
4 gm. of the kernels, although other feed was withheld for a day. We 
concluded from this that even the 4 gm. had affected it physiologically 
and had made it suspicious of the feed. After a week upon control feed, 
it ate feed 316 without objection. 
Rabbit 957, which had eaten feed 316 for 46 days without noticeable 
effect, was rested for three weeks and then fed the residue after petro¬ 
leum-ether extraction, which does not remove the gossypol. Its appetite 
was perceptibly affected on the third day, but it ate most of the feed for 
6 days. On the ninth day it refused to eat feed 290, but ate green feed 
slowly. It died on the fourteenth day, showing symptoms of cottonseed- 
meal poisoning. See Table I. 
Table I.— Results of feeding cottonseed kernels (fat-free; feed 290) and cottonseed meal 
to rabbits and guinea pigs 
Weight of animal. 
Weight of feed 
eaten. 
Num¬ 
ber of 
days 
fed. 
Feed and animal No. 
Initial. 
Final. 
Loss. 
Actual. 
As 
ker¬ 
nels. 
Result. 
Cottonseed kernels: 
Rabbit 958. 
Gm. 
1,560 
680 
Gm. 
r » 430 
Gm. 
130 
Gm. 
56.5 
0 
Gm. 
85 
0 
II 
Made sick and re¬ 
fused to eat. 
Refused feed en¬ 
tirely. 
Refused the feed. 
Died. 
Guinea niff A 
I 
Guinea pig B. 
650 
I, 806 
4 
6 
1 
Rabbit <K7. 
1.535 
1.238 
235 
339 
IOO 
150 
14 
13 
Cottonseed meal : a 
Average for 22 
rabbits. 
h 577 
6 133 
All died. 
a The results of Withers and Brewster’s experiments (1913) with cottonseed meal are here inserted for 
comparison. 
& Each rabbit consumed from 48 to 225 gm. of cottonseed meal and died upon the feed in from 6 to 22 days. 
TOXICITY OF GOSSYPOE EXTRACT 
It is much simpler to prepare gossypol from cottonseed than from 
the oil. 1 Qualitative tests of ground cottonseed showed that gossypol 
could be extracted with ether, carbon bisulphid, chloroform, benzene, 
alcohol, but not with petroleum ether or gasoline. By extracting the 
1 This point will be discussed under the chemistry of gossypol, which will appear in a subsequent pub¬ 
lication. 
