Nov. 15,1915 Gossypol, the Toxic Substance in Cottonseed Meal 269 
Rabbit 928.—Fatal in three hours. Excess discolored serous fluid in abdomen 
containing oily globules. Moderate injection in intestines at points. Slight excess of 
fluid. Lungs slightly congested and slightly edematous. 
Rabbit 929.—Died during night between the second and the thirteenth hour. 
Excess brownish serum in abdominal cavity. Small intestines show areas of marked 
injection. Lungs congested and somewhat edematous. 
Rabbit 93 o.—Slightly indisposed on following day and normal thereafter. Appetite 
only slightly affected. 
Rabbit 933.—Same as 930. 
Feeding Gossypol Extract with Corn Mead and Molasses 
An artificial cottonseed meal was made by pouring the concentrated 
ether extract of cottonseed kernels over corn meal. The daily feed for 
each of four rabbits was estimated to be equivalent to 30 gm. of cotton¬ 
seed kernels, and for each of two others, 15 gm. Control animals were 
given corn meal and molasses. All the animals were supplied liberally 
with green feed (pea vines, cabbage, and collards) in the morning. In 
the afternoon (4 or 5 p. m. J they were given the various feeds mixed with 
molasses. The controls on corn meal and molasses did well, gained in 
weight, and need not be further mentioned. The gossypol extract 
proved Very toxic. The animals receiving the equivalent of 30 gm. of 
cottonseed kernels refused to eat the cottonseed feed after the fifth day. 
They began to refuse green feed later, became sicker, and the last one 
died within 15 days. The two rabbits and a guinea pig receiving smaller 
doses were soon made sick. One rabbit and a guinea pig refused the feed 
thereafter, and the other rabbit died. See Table IV. 
Table IV.— Results of feeding gossypol extract (feed 318) with corn meal and molasses 
to rabbits and guinea pig a 
DAILY REED EQUIVALENT TO 30 GM. OP COTTONSEED KERNELS 
Peed and animal No. 
Weight of animal. 
Weight of mix¬ 
ture eaten. 
Num¬ 
ber of 
Result. 
Initial. 
Pinal. 
Eoss. 
Actual. 
As ker¬ 
nels. 
days 
fed. 
Gossypol extract 
with corn meal 
and molasses: 
Rabbit 941... 
Gm. 
I » S 3 S 
Gm. 
L 255 
Gm. 
280 
Gm. 
52 
Gm. 
104 
8 
Died. 
Rabbit 942,.. 
1,605 
1,250 
355 
54 
108 
12 
Do. 
Rabbit 943... 
I> 53 ° 
x, 180 
350 
70 
140 
II* 5 
Do. 
Rabbit 944... 
2,095 
L 595 
5 °o 
. 7 i 
142 
15 
Do. 
Average... 
1,691 
1,320 
37 i 
62 
124 
11. 7 
Do. 
<* 1 gm. of the mixture of feed 318 and dry com meal is equivalent to approximately 2 gm of cottonseed 
kernels. 
