270 
Journal of Agricultural Research 
Vol. V, No. 7 
Table IV .—Result of feeding gossypol extract (feed 318) with corn meal and molasses 
to rabbits and guinea pig —Continued 
DAILY FEED EQUIVALENT TO 15 GM. OE COTTONSEED KERNELS 
Weight of animal. 
Weight of mix¬ 
ture eaten. 
Num- 
4j ___ 4 4 | « v 
ber of 
Result. 
Jr ecu aiiQ animal JN o» 
days 
Initial. 
Final. 
Loss. 
Actual. 
As ker¬ 
nels. 
fed. 
Gossypol extract 
with com meal 
and molasses: 
Gm. 
Gm. 
Gm. 
Gm. 
Gm. 
Rabbit 953... 
1*9*5 
i } 755 
160 
41 
82 
II 
Died. 
Rabbit 954... 
1, 790 
1,740 
50 
80 
160 
20 
Experiment discon- 
tinued. 
Gossypol extract 
alone: 
Guinea pig A 
770 
650 
120 
34 
68 
29 
Do. 
POST-MORTEM OBSERVATIONS 
Rabbit 941.—Reddish serum in abdominal cavity, 15 c. c. Cecum deeply injected. 
Liver congested. Lungs slightly congested and edematous. Conspicuous thrombus 
in right auricle. 
Rabbit 942.—Excess abdominal fluid, 15 c. c. Hemorrhagic (inflamed) and ulcer¬ 
ated condition at pyloric end of small intestines. Large thrombus in right auricle. 
Rabbit 943.—Slight excess of abdominal fluid. Large intestines had some hemor¬ 
rhagic areas. Liver congested. 
Rabbit 944.—Reddish serum in abdomen, 25 c. c. Serous membrane injected. 
Small intestines reddened. Small thrombi present. Death due to enteritis. 
Rabbit 953.—Mesenteric blood vessels injected. Viscera practically normal. 
Liver much congested. Kidneys much congested. 
Rabbit 954.—Experiment discontinued because animal refused to eat feed 318 
after the eighth day. Subsequently put on precipitated gossypol. 
Guinea pig A.—Experiment discontinued because animal refused to eat feed 318. 
In order to ascertain the effect of a large dose, a large healthy rabbit 
(945) was taken from the control feed and given all of feed 318 that it 
would eat. It consumed 40 gm., equivalent to 80 gm. of kernels, on the 
first day and was made sick on the following day. When it began to 
recover on the fourth day it was given a small feed and died on the 
ninth day, having lost considerably in weight. The protocol of rabbit 
945 is as follows: 
September 23, p. m.—Ate 40 gm. of feed 318 with molasses. 
September 24.—Appears sick; has diarrhea. Ate little green; refuses feed 318. 
September 25.—Seems indisposed; refuses feed 318. 
September 26.—Better; eats cabbage. Weight 2,700 gm. Given 15 gm. of feed 318 
and 15 gm. of corn meal with molasses. Ate equivalent to 7 gm. of feed 318. 
September 27, 28, and 29.—Eats pea vines readily. 
September 30.—Refuses green; p. m., ate com meal and molasses readily. 
October i, a. m.—Refuses green. Died ninth day about 3 p. m. Weight, 2,410 gm. 
Post-mortem examination showed considerable excess fluid in abdominal cavity. 
