274 
Journal of Agricultural Research 
Vol. V, No. 7 
To prepare the injection, 0.7 gm. of this substance was dissolved in 
ether and the ethereal solution mixed with 20 c. c. of purified cottonseed 
oil. The clear reddish yellow solution was warmed over steam until it 
had not the slightest odor of ether. This was then injected in doses of 
10 c. c. into two rabbits, 963 and 964, weighing 1,560 and 1,485 gm., 
respectively. In a few minutes the rabbits became very uneasy and 
then passed into a sort of stupor. Rabbit 963 died in 3.5 hours and 964 
in 4.5 hours. The death of rabbit 964 was witnessed. Shortly before 
death it toppled over on its side, had several convulsions, gasped several 
times, squealed, and died. 
In these cases, as in the previous one, there was considerable injecta 
left in the abdominal cavity. See Table VI. 
Table VI .—Result of administering crystalline gossypol “acetate ” intraperitoneally in 
cottonseed oil to rabbits 
CRYSTALLINE GOSSYPOL “ACETATE” 
Rabbit No. 
Initial 
weight of 
rabbit. 
Weight of 
gossypol. 
Dose 
volume. 
Weight of 
gossypol 
per kilo 
of body 
weight. 
Result. 
QCC. 
Gm. 
1,115 
I, 180 
Gm. 
0. 55 
•55 
C. c. 
8 
Gm. 
0-493 
.466 : 
Died. 
qc6. 
8 
Do. 
y j . 
recrystallized gossypol “acetate” 
963. 
1,560 
o- 3 S 
10 
0.244 
Died. 
964. 
1.48s 
• 35 
10 
• 235 
Do. 
POST-MORTEM OBSERVATIONS 
Rabbits 955 and 956.—Dead and cold after six hours. Apparent nonabsorption of 
much of the injection. Excess of fluid. Peritoneum stained brown. Visceral blood 
vessels slightly injected. 
Rabbit 963.—Died in convulsions. Part of injecta present as oily globules. Serum 
present also. Serous membrane stained yellow. 
Rabbit 964.—Same as 963, except small intestines were rather markedly injected. 
Feeding Crystalline Gossypol “Acetate” to Rabbits 
Crystalline gossypol “acetate” with corn meal and molasses (feed 
319) was fed to rabbit 965. The feed was refused on the fourth day, after 
which it was not further given. Only on the first day did the animal 
eat the entire amount fed. After eating 0.3 gm. of crystallized gossypol 
“acetate,” it had a bad diarrhea add little appetite for green feed the next 
day. The protocol was as follows: 
December 15, first day.—Ate 0.3 gm. with com meal and molasses; weight, 2,340 
gm. 
December 16, second day.—Bad diarrhea, and eats little green feed. 
