276 
Journal of Agricultural Research 
Vol. V, No. 7 
tion. Gossypol from old seeds is greenish. The post-mortem examina¬ 
tion showed considerable irritation in the small intestines of these 
rabbits. 
EFFECT OF SMALL DOSES OF CRYSTALLINE GOSSYPOL “ ACETATE ” 
Rabbit 978, weight 2,100 gm., was fed on recrystallized gossypol 
“ acetate ” at the rate of 0.05 gm. daily mixed with corn meal and molasses. 
After one week it began to show a diminished appetite for the feed. On 
the nineteenth day it was given a double dose by mistake, and for two 
weeks thereafter showed a very poor appetite for the feed. At that time 
it weighed 1,820 gm. 
Its record by weeks is as follows: 
Quantity of gossypol eaten.gm.. 0.34; 0.33; 0.335; 0.25; 0.25; 0.32; 
0.125. 
Weight of rabbit.gm.. 2,045; 2 >°io; 2,070; 1,820; 1,930; 
1 . 730 - 
Total quantity of gossypol eaten.gm.. 1.95. 
The animal died after 51 days. The post-mortem examination showed 
that a convolvulus had set up a necrotic condition in the intestine. 
Whether the feed was contributory to this condition we are unable to 
say. See Table VII. 
Table VII .—Results offeeding crystalline gossypol “acetate ” (feed 31 p) to rabbits 
Weight of rabbit. 
Weight 
of gos¬ 
Num¬ 
ber of 
days 
fed. 
Result. 
Feed and rabbit No. 
Initial. 
Final, 
Loss. 
sypol 
“acetate” 
eaten. 
Gossypol “acetate”: 
Gm. 
Gm. 
Gm. 
Gm. 
Made sick. 
965. 
2,340 
2, 140 
200 
O. 67 
5 
95 i. 
1, 800 
1,170 
630 
.92 
28 
Died. 
965A. 
2,265 
I, 600 
665 
2 - 53 
55 
Do. 
Recrystallized gossypol 
“acetate”: 
974 . 
1,670 
340 
330 
•47 
i 5 
Made sick. 
976. 
1,670 
h 195 
475 
.40 
i 5 
Died. 
977 . 
1,825 
h 2 45 
580 
•35 
13 
Do. 
97 2 .;■ 
1,42s 
ijiiS 
310 
•55 
13 
Do. 
Gossypol ‘ ‘ acetate fed 
in small doses (0.05 
gm. per day): 
Died from second¬ 
ary causes. 
978 . 
2 , IOO 
1.730 
380 
i* 95 
5 1 
Feeding Gossypol “Acetate” to Fowls 1 
Two cockerels (986 and 987) previously fed on cottonseed meal to 
study the symptoms, were started on gossypol. Powdered gossypol 
in 0.3 gm. doses was fed, followed by a little water. On the fourth 
1 This experiment was carried on under the supervision of Dr. B. F. Kaupp, Pathologist, of the Poul¬ 
try Division, North Carolina Agricultural Experiment Station. 
