282 
Journal of Agricultural Research 
Vol. V, No. 7 
removed and boiled in a large flask with alcohol. The first alcoholic 
extracts were quite highly colored. The extraction was repeated until 
a filtrate was obtained which possessed only a pale-yellow color. 
The ether-alcohol-extracted kernels were fed daily to three rabbits for 
from 72 to 105 days in amounts ranging from the equivalent of 15.2 to 
24 gm. of kennels; at the end of the period the rabbits were normal and 
all had gained from 30 to 148 per cent of their initial weight and were 
still gaining. 
The severe test that these rabbits endured is sufficient to show that a 
feed has been prepared which can be called practically nontoxic. 
It also indicates that protein and organic phosphates (inosite phos¬ 
phoric acid salts), which are present in the feed in larger amounts than 
in cottonseed meal, have very little, if anything, to do with cottonseed- 
meal poisoning. 
Table XI.— Results of feeding cottonseed kernels extracted with gasoline , ether , and 
alcohol (feed 331) to rabbits 
Rabbit No. 
Weight of rabbit. 
Weight 
of feed 
eaten. 
Equivalent of feed 
eaten as kernels. 
Number of 
days fed. 
Initial. 
Final. 
Gain. 
Total. 
Daily. 
066. 
Gm. 
i. 335 
640 
I, 610 
Gm . 
1,897 
1.590 
2.095 
Gm. 
562 
950 
485 
Gm. 
1,043 
957 
1,108 
Gm. 
h 738 
1.595 
I, 846 
Gm. 
24 
i 5 - 2 
19. 6 
72 
105 
94 
O67. 
968. 
Rabbit 967 was slightly off its feed only on the fortieth and forty-first 
days, but recovered quickly and continued to gain. See Table XII. 
Rabbit 968 was one of the lot of Belgian hares received from Washing¬ 
ton, D. C., in rather poor health. It was started at the rate of 15 gm. 
daily, equivalent to 25 gm. of whole kernels. This proved too heavy 
feeding, for after two weeks the animal went off its feed for several 
days. The ration was then reduced (Table XII). 
\ Table XII.— Record of rabbits q6 7 and q68 on feed 331 
Rabbit No. and period 
(10-day). 
Weight of Weight of 
feed eaten. rabbit. 
Rabbit No. and period 
(10-day). 
Weight of 
feed eaten. 
Weight of 
rabbit. 
Gm* 
Gm. 
Rabbit 967. 
1 . 
2 . 
3 . 
4 . 
5 . 
6 . 
7 . 
8 . 
9 . 
10 . 
Last 5 days... 
62 
70 
73 
93 
61 
96 
100 
100 
122 
120 
60 
640 
7<>5 
845 
93 ° 
a L 055 
0 1, 200 
a i ,335 
a 1, 420 
a L5i5 
a 1, 5^0 
a 1, 59 ° 
Rabbit 968 
Gm. 
1 . 
2 . 
3 . 
4 . 
5 --. 
6 . 
7 . 
8 ....,. 
9 .•. 
Last 4 days... 
140 
85 
81 
100 
100 
107 
i35 
150 
150 
60 
Gm. 
I, 610 
L 5 2 5 
a 1,410 
a 1,460 
a 1, 640 
0 i, 725 
a 1, 900 
a 2,105 
a 2,130 
a , 6 1, 890 
2,095 
a 10 gm. of com meal was added daily to the feed. 
b L oss i n weight was due to the delivery of seven young rabbits. 
