Dec. 13,1915 
Translocation of Constituents of Seeds and Tubers 453 
As the growth of the seedlings proceeded, the cotyledons began to 
shrink and finally turned brown. The root development in all cases 
was good, nearly filling the test tubes, and each seedling developed two 
perfect leaves. The seedlings were allowed to grow until they began to 
etiolate and wilt, this period being reached in from 17 to 22 days. The 
plants thus grown were very uniform in size and development, the aver¬ 
age height being 6^2 inches. During their development care was taken 
that they should not touch each other. As fast as they matured, they 
were removed from the test tubes and the cotton carefully removed 
from the stem and roots. The plants were then divided into roots (8), 1 
lower stems (5) which averaged 4X inches in height, exhausted cotyledons 
(7), upper stems (6) which averaged 2 inches, and the leaves (4). The 
liquid remaining in the test tubes was evaporated to dryness and added 
to the washings (11). 
Six hundred and nine selected beans labeled “B” received the same 
treatment as those labeled “A,” except they were allowed to live only 
until the radicle had appeared and the integument had softened. The 
integument (2) and the cotyledons (3) were carefully air-dried, as were 
the above-mentioned plants. The drainage and washings (1) from these 
beans were carefully evaporated to dryness* These several parts of the 
beans were analyzed to check the analyses of the seedlings, the results of 
which are given in Table I. 
In analyzing fhe separate portions of the air-dried material which had 
been carefully ashed at a dull-red heat, three portions of 0.2000 gm. 
each were carefully weighed out. In one portion phosphorus and silica 
were determined, while in another portion the determination of potassium 
was made. The methods used were essentially the official methods of 
the Association of Official Agricultural Chemists. 2 In a third portion 
of the ash, calcium and magnesium were determined according to the 
method of McCrudden. 3 
In Table I are to be found the results of the analyses of the separate 
portions of 609 seedlings and the separate parts of 609 beans. 
It is evident from the results given in Table I that the weight of the 
total ash of the seedlings agrees fairly well with the total weight of the 
ash of the bean control, the difference being due in all probability to 
unavoidable outside contamination during the period of growth. The 
comparative analyses of the inorganic constituents fall well within the 
limit of experimental error. The greatest difference is observed in the 
case of silica, the seedlings containing nearly twice as much as the beans. 
1 The numbers in parentheses refer to the number of part in the tables. 
2 Wiley, H. W., et al. Official and provisional methods of analysis. Association of Official Agricultural 
Chemists. As compiled by the committee on revision of methods. U. S. Dept. Agr. Bur. Chem. Bui. 107 
(rev.), 272 p. t 13 fig. 1908. 
* McCrudden, F. H. The quantitative separation of calcium and magnesium in the presence of phos¬ 
phates and small amounts of iron devised especially for the analysis of foods, urine, and feces. In Jour. 
Biol. Chem., v. 7, no. 2, p. 83-100. 1910. 
-Hie determination of calcium in the presence of magnesium and phosphates: the determination of 
calcium in urine. In Jour. Biol. Chem., v. 10, no. 3, p. 187-199. 1911. 
