468 
Journal of Agricultural Research 
Vol.V, No. ii 
Five days later, water-soaked spots appeared on the leaves, and by 
November 3 there were typical browned spots on plants inoculated with 
each of the three colonies. These spots swarmed with bacteria. Poured 
plates on agar gave pure cultures of the same white organism. No further 
inoculations were made until April 30, 1915, when sprayings were again 
made in cages as before, using subcultures of colony No. 1, plated from 
a spot produced by the inoculations of October 26. The plants used in 
this case were of a common field variety and rather stunted but with 
sound leaves. Three days after the first spraying water-soaked spots 
appeared on the lower surface of the leaves, and by May 6 these had 
enlarged into the typical angular, dry, brown spots. 
Another experiment on May 6, 1915, using perfectly healthy, free- 
growing Arlington white spine cucumber plants and subcultures from the 
same colony (No. 1) gave striking results. Several leaves showed tiny 
water-soaked areas on the second day, and all the leaves were typically 
and badly spotted by the sixth or seventh day. In this stage the spots 
were one-fourth to three-fourths of an inch in diameter, angular, following 
the larger veins, and water-soaked (translucent), not dry. In the early 
morning drops of moisture (exudate) swarming with bacteria were found 
hanging on the lower surface of such spots (PL XLV, fig. 1). Pure 
cultures of the causal organism were obtained by plating from one of these 
drops. On the following day, or even later on the same day, white 
films (bacterial crusts) replaced the drops (PL XLIII, fig. 1). The appear¬ 
ance of infected leaves at the end of 12 to 14 days, when the diseased 
areas have become dry and begin to drop out, is shown in Plate XLIII, 
figure 2. 
As the young unsprayed leaves developed on these plants, they 
became naturally infected; and in three cases the stems and petioles of 
this young growth also became water-soaked, exuded drops of fluid 
(Pl. XLIV, X, X), and finally broke or bent over (PL XLV, fig. 2), ending 
the growth of the plant. The cracking open of stems in this stage of the 
disease is shown at X in Plate XLV, figure s, and in detail in Plate XLV, 
figure 3. 
On the green fruits up to the end of August, 1915, the writers were 
able, with one exception, to obtain within a week or 10 days (shipping 
time) only a local infection and a bacterial exudate such as that shown in 
Plate XLVI, figure 1—no general soft-rot. Even when the fruit (PL 
XLVI, fig. 1) was kept for another week at high temperatures (28° to 32 0 
C.), it did npt rot (Pl. XLVI, fig. 2). Altogether 15 such fruits were 
inoculated with virulent cultures, some on the vines and others in damp 
chambers. 
Soft-rot occurred twice in young fruits (two-thirds grown) when placed 
in damp chambers after inoculation. In the first case (the exception 
referred to above), plates were poured from the soft interior of the one 
fruit thus affected. As only spreading fimbriate colonies were obtained, 
the soft-rot was attributed to an intruder, and no further studies were 
