530 
Journal of Agricultural Research 
Vol. V, No. 12 
In order to understand more fully the different tabulations, a brief 
description of each sample follows. 
Nos. 285, 812, and 852 were collected in April of 1912, 1913, and 1914, 
respectively, from the cut end of the same main branch about 20 feet 
from the root of the vine and just after the sap flow commenced. 
No. 853 was collected in April, 1914, from the cut end of another main 
branch about 4 feet from the root of the vine and just after the sap flow 
commenced. This sample was taken at the same time as No. 852. 
No. 854 was collected in April, 1914, from the same point as No. 852, 
but seven days later and just before the sap flow ceased. 
No. 900 was collected in April, 1915, from the cut end of one of the main 
branches about 20 feet from the root of the vine and just after the sap 
flow commenced. This was a different branch from that from which No. 
285 was taken, because no sap exuded from the old branch, and it seemed 
to have been greatly weakened by the annual loss. 
No. 901 was collected in April, 1915, from several of the small branches 
or shoots which were of the previous year's growth and just after the sap 
flow commenced. This sample was taken at the same time as No. 900 
and from 10 shoots which were located several feet from the main branches. 
Nos. 902, 904, and 906 were collected for three successive days from 
9 a. m. to 5 p. m., beginning on April 29, 1915, four days after and from 
the same point as No. 900. 
Nos. 903, 905, and 907 were collected for three successive nights from 
5 p. m. to 9 a. m., beginning on April 29, 1915, and from the same point 
as No. 900. 
The variation in the percentage composition of the fresh sap and the 
ash of samples 852, 853, 900, and 901 are given in Tables I and II. 
Table I .—Variation in percentage composition of fresh sap collected at the same time 
from different points on the wild-grape vine 1 
Constituent. 
Sample 
No. 852. 
Sample 
No. 853. 
Sample 
No. 900. 
Sample 
No. 90X. 
Ratio between— 
Nos. 852 
and 853. 
Nos. 900 
and 901. 
Water at ioo° C. 
99.8279 
S> 9*8538 
99.8183 
99-8431 
1 rx.oo 
x : 1.00 
Organic matter. 
*1435 
. 1112 
■1305 
.1068 
1 : .77 
x: .82 
Silica (SiOs).. 
.0001 
.0001 
.0003 
.0017 
1:1.00 
x : 5.67 
Ferric and aluminic oxids (FesOs+ 
AlsOs). 
.OOOl 
.0001 
.0001 
.0004 
1:1.00 
x: 4.00 
Calcium oxid (CaO). 
.0160 
•0155 
.0234 
.0268 
x : .97 
x : 1.15 
Magnesium oxid (MgO). 
.0024 
• 002s 
.0041 
.0062 
1: 1.04 
1:1. si 
Sodium oxid (NaaO). 
.0012 
.00x2 
.00x0 
.00X1 
1: 1.00 
1 : x. 10 
Potassium oxid (KaO). 
.0050 
.01X2 
.0167 
• 0074 
x : 2.24 
1: .44 
Phosphorus pentoxid (P2O5). 
.0015 
.0026 
.0030 
.0030 
x : 1.73 
1: 1.00 
Sulphur trioxid (SOs). 
.0019 
.0017 
.0025 
.0033 
i: .89 
1: 1.32 
Chlorin... 
.0004 
.OOOI 
.0001 
.0002 
x : .25 
1: 2.00 
Total . 
100* 0000 
100* 0000 
xoo. 0000 
xoo. 0000 
1.0009 
1.0008 
1.00082 
1.00027 
1: 1.00 
1: 1.00 
. 
*5 
Nitrogen as nitrates. 
.0013 
.0024 
.00004 
. 00001 
1: 1.85 
x : .25 
Crude ash— .. 
.0384 
.0477 
. 0700 
.0662 
x : x. 24 
1 : -95 
1 Nos. 852 and 853 were collected in 1914; Nos. 900 and 901 in 191s- 
