564 
Journal of Agricultural Research 
Vol. V, No. 13 
An attempt was made to accentuate the effect of the diuretic on the 
milk flow by keeping the water intake at a level which under normal 
conditions would be entirely sufficient for the animal, but with the addi¬ 
tional requirements during diuresis draw heavily upon the body fluids. 
Goat 1 was used as the experimental animal. See Table III. 
TabL 3 III .—Effect of urea with constant level of water intake on milk flow of goat 1 
Bate. 
Water. 
Urine. 
Milk. 
Solids. 
Fat. 
Nitro¬ 
gen in 
5 c * c * 
Remarks. 
Jan. 26.. 
C. c. 
2, OOO 
C. c. 
7 I 5 
C. c. 
780 
Per cent. 
15. 06 
Pr. ct. 
5-5 
Mgm . 
33-9 
No additions to ration. 
27 .. 
2 , OOO 
640 
840 
15. 06 
5-4 
33 * 2 
Do. 
28 .. 
2 , OOO 
815 
695 
16.19 
6.7 
33.0 
Collection from 30 gm. of 
20. . 
2 , OOO 
550 
860 
15. 70 
6.7 
29.6 
nrea. 
Do. , 
30. . 
2 , OOO 
675 
900 
15.06 
5.5 
32.8 
Do. 
3 l0 ‘ • 
2 , OOO 
260 
845 
i 5 - 99 
5-9 
33-6 
No additions to ration. 
Feb. 1.. 
2 , OOO 
85s 
72s 
16.18 
5-9 
35 - 7 
Do. 
2 .. 
2 , OOO 
35° 
820 
15 . 08 
5-7 
35 - 1 
Do. 
a Animal very restless; beets not all consumed. 
As seen in Table III, renal activity after the effects of the first day 
.was not sufficient to draw noticeably on the mammary secretion for 
fluids. At no time when urea was given, even in the above experiment, 
did the animal show any abnormal desire for water; in fact, the water 
supply when replenished in the morning was usually left untouched for 
some time. Yet it is hardly to be questioned that the animal was in 
great need of water. On the morning of February 2, after the previous 
day's collection had been made, 25 gm. of urea were given in one dose 
to determine whether larger quantities of urea were necessary to produce 
the desired results. This amount of urea, while large, would not furnish 
any more urea for excretion through the kidneys than 70 gm. of protein, 
and no untoward effects were expected. Yet five minutes after the urea 
was given the animal lay down and soon passed into violent convulsions, 
which terminated fatally in 1 hour and 15 minutes. A morphine hypo¬ 
dermic was of no avail in preventing death. A post-mortem examination 
gave no clue to the cause of death. Apparently the maximum quantity 
of urea which could possibly be retained with safety in the circulation 
had accumulated during the previous period of urea administration. 
With the sudden flooding of the system with the additional 25 gm. of 
urea the safety limits were exceeded and death resulted. Immediately 
previous to the administration of the final dose of urea the animal was 
ruminating and apparently normal in all respects. The urea used was a 
Kahlbaum preparation and undoubtedly was free from such other toxic 
compounds as cyanid or cyanate, as no ufitoward results followed the 
subsequent use of urea from the same reagent bottle. It was barely 
