66 o 
Journal of Agricultural Research 
Vol. V, No. is 
storage after the ninth day of refrigeration. Only one egg hatched on 
the ninth day, and but 2 out of 2,327 removed on the seventh, eighth 
and ninth days. After the tenth to fifteenth days of refrigeration, 
2,221 eggs were removed to warmer temperature, but none hatched. 
Mortality increased rapidly after the fourth day of refrigeration; thus, on 
the fifth day only 15 out of 735 eggs hatched. (See Table I.) 
Table I .—Effect of cold-storage temperatures upon eggs and larvae of the Mediterranean 
fruit fly 
Number of days in 
cold storage. 
Tempera¬ 
ture of 
storage 
room. 
Eggs. 
Larvae. 
Number 
under 
observa¬ 
tion. 
Num¬ 
ber 
hatch¬ 
ing 
after re¬ 
moval 
from 
storage. 
First instar. 
Second instar. 
Third instar. 
Num¬ 
ber 
alive. 
Num¬ 
ber 
dead. 
Num¬ 
ber 
alive. 
Num¬ 
ber 
dead. 
Num¬ 
ber 
alive. 
Num¬ 
ber 
dead. 
°F. 
1 .. 
32 
8f 
81 
252 
40 
33 
7 
2. 
32 
528 
520 
94 
O 
463 
9 
53 
2 
3 . 
32 
150 
135 
37 
I 
226 
15 
16 
75 
4 . 
32 
336 
216 
285 
26 
152 
O 
101 
3 
c. 
32 
735 
IS 
196 
202 
71 
175 
6. 
32 
469 
12 
26 
165 
18 
5 ° 
105 
10 
7 . 
32 
659 
I 
11 
454 
14 
64 
135 
132 
8;. 
32 
834 
0 
2 
84s 
20 
423 
38 
200 
9 . 
32 
734 
I 
0 
339 
II 
473 
20 
429 
10. 
32 
0 
701 
0 
257 
11. 
32 
63s 
O 
0 
450 
0 
332 
6 
374 
12. 
32 
887 
O 
0 
440 
0 
493 
0 
157 
13. 
32 
0 
355 
0 
276 
0 
173 
14 . 
32 
699 
O 
0 
273 
0 
248 
0 
152 
ic. 
32 
0 
262 
0 
144 
j . 
2. 
32-33 
86 
0 
78 
0 
3 
0 
3 . 
32-33 
154 
1 
146 
2 
89 
0 
4 .. 
32-33 
46 
0 
73 
0 
32 
0 
5 . 
32-33 
, 96 
0 
39 
0 
30 
0 
6. 
32-33 
152 
23 
279 
7 
8 
1 
24 
0 
7 . 
32-33 
3 i 
1 
16 
11 
9 
0 
8.. 
32-33 
401 
5 
35 
163 
3 
27 
10 
16 
9 . 
32-33 
0 
169 
0 
167 
2 
14 
10. 
32-33 
357 
0 
2 
179 
0 
no 
0 
3 i 
12. 
' 32-33 
784 
0 
0 
880 
0 
86 
0 
35 
*3 . 
32-33 
900 
0 
0 
637 
0 
35 
0 
2 
14. . 
32-33 
1, 001 
0 
0 
425 
0 
42 
0 
28 
32-33 
1,121 
0 
0 
255 
16.. 
22-2* 
312 
0 
0 
Ciq 
0 
42 
37 . 
32-33 
0 
143 
0 
29 
0 
3 
3 . 
33-34 
60 
0 
94 
0 
55 
0 
4 . 
33-34 
108 
2 
107 
2 
68 
0 
c. 
33-34 
42 
26 
79 
28 
6. 
33-34 
68 
32 
286 
169 
8 
5 
7 . 
33-34 
75 
20 
81 
100 
55 
1 
8. 
33-34 
3 00 
45 
46 
20 
35 
175 
5 i 
48 
9 . 
34-34 
500 
0 
38 
207 
48 
45 6 
31 
189 
10. 
33-34 
54 i 
0 
4 
1,446 
32 
296 
0 
48 
11.. 
33-34 
0 
72 
0 
3 r 4 
4 
126 
12. 
33-34 
358 
0 
1 
215 
0 
509 
0 
48 
13. 
33-34 
2 
632 
0 
38s 
0 
4 
