Jan. xo, 1916 
Mature Beef and Immature Veal 
685 
Solutions. —Digestions were made in 0.2 per cent hydrochloric-acid 
and in 0.5 per cent sodium-carbonate solutions. 
Enzym preparations. —The following preparations, all in powder 
form, were used: 
Pepsin 1: A 100-gm. bottle of pepsin (1:3,000), Parke, Davis & Co.; 
purchased about May, 1912. 
Pancreatin 1: A 1-ounce bottle of pancreatin (Parke, Davis & Co.); 
an old preparation. 
Trypsin 1: A 1-ounce bottle of trypsin (Merck); purchased in Sep¬ 
tember, 1912. 
Trypsin 2: A 200-gm. bottle of trypsin sicc. (Greubler); imported about 
March, 1912. 
Trypsin 3: A50-gm. bottle of trypsin (Merck); purchased in August, 
1913 - 
In every case the unopened bottle of enzym preparation was used. 
Portions were transferred to weighing bottles and dried for several days 
in desiccators until the loss in weight was slight. The bottles were then 
stoppered. The day before being used the enzym preparations were 
dried in a desiccator and portions weighed as needed. 
In order to correct the digestion data for nitrogen introduced in the 
form of enzym, their nitrogen contents were determined. The results 
are summarized in Table VII. The methods used were similar to those 
employed throughout the work. 
Table VII .—Quantity of Nj 5 nitrogen per gram of dry enzym preparation 
Preparation. 
Total nitrogen. 
Ammonia ni¬ 
trogen. 
Amino nitro¬ 
gen. 
Pepsin 1. 
C. c. 
51 - I 
40. 9 
47-3 
70. 1 
47 * 0 
C. Ct 
C. c. 
Pancreatin 1. 
Trypsin 1. 
None. 
62. 5 
1. 0 
Trypsin 2. 
2 . O 
22 . 8 
Trvosin 2.. 
All of the digestion experiments were begun with freshly hashed beef 
or immature veal, except experiments 5 to 8, in which powdered meats 
beef sample 1.1 and veal sample 1.1, were used. These were prepared 
as follows: 
Veal sample 1.1: Seven kgm. of veal sample 1 were hashed and trans¬ 
ferred to two 8-liter wide-mouth bottles. Seven liters of 50 per cent 
alcohol were added and the mixture well stirred. After 24 hours the 50 
per cent alcohol was strained off through cheesecloth and replaced with 
an equal volume of 75 per cent and the next day with 95 per cent alcohol 
This was followed by two treatments with 2 liters of absolute ether. 
The ether was removed by straining through cheesecloth and squeezing 
