Jan. io, 1916 
Mature Beef and Immature Veal 
697 
nitrogen determinations (see p. 696). The results for amino nitrogen in trypsin 2 
were obtained by introducing a solution of the enzym in o. 5 per cent sodium carbonate 
directly into the amino-nitrogen apparatus. The ammonia nitrogen present in this 
preparation reacted completely with nitrous acid in the 20 minutes’ reaction period 
used uniformly in the determinations. The small amount of ammonia nitrogen present 
in trypsin 3 permitted the determination of amino nitrogen in the residue obtained 
after ammonia removal and the use of this figure as the correction (see TableV II). 
The conditions of experiments 26 to 34 were as follows: In each experiment the 
digestion mixture consisted of 100 gm. of meat plus 2,000 c. c. of 0.5 per cent sodium- 
t*ig. 3.—Experiment 28. Curve showing the quantity (in cubic centimeters) of Nfs nitrogen in 100 c. c. of 
digestion fluid, equivalent to approximately 5 gm. of meat or 30 gm. of skim milk; used 100 gm. of veal 
sample 9 or 600 gm. of skim-milk sample 2, 2,000 c. c. of 0.5 per cent sodium carbonate, and 2,000 gm. of 
trypsin 2. 
carbonate solution plus the amount of trypsin indicated in Table XII. For nitrogen 
determinations, 100 c. c. digestion fluid, equivalent to approximately 5 gm. of meat, 
were used. 
Table XI.— Rate of formation of proteoses , peptones , and amino adds in trypsin-sodium- 
carbonate solution 
QUANTITY (IN CUBIC CENTIMETERS) OP Nfj PROTEOSE, PEPTONE, AND AMINO-ACID NITROGEN 
Experiment No. — 
Digestion period. 
26® 
25 6 
27 
28 
30 
31 
32 
34 
Beef sam¬ 
ple 8. 
Veal sam¬ 
ple 8. 
Beef sam¬ 
ple 8. 
Veal sam¬ 
ple 8. 
A 
sample 2. 
1 
S Os 
<31 
la 
> 
Skim-milk 
sample 2. 
r 
85 o* 
> 
Beef sam¬ 
ple 10. 
1 
| 0 
(U 
a ■a 
> 
Beef sam¬ 
ple 10. 
Veal sam¬ 
ple 10. 
Beef sam¬ 
ple 11. 
Veal sam¬ 
ple 11. 
Beef sam¬ 
ple 12. 
Veal sam¬ 
ple 12. 
Hours. 
2. 
22.2 
. 18.2 
. 17.0 
18.2 
- 44-3 
9-3 
41.8 
10.4 
14.7 
13-7 
9-7 
11.4 
18.4 
. 18.4 
.21.5 
19.7 
6. 
32.8 
26.8 
25 -3 
28.2 
46. 2 
19.9 
45-2 
20.5 
27.0 
27. 2 
19.0 
21. s 
3 i *7 
33*8 
34*7 
34*3 
Theoretical 
maximum 
Rytra rtivi 1 
S 2.4 
41.9 
SO -5 
41.8 
47.1 
45*3 
45-6 
44-5 
49-3 
4 i -3 
49-1 
40.1 
51*2 
45*6 
5 i *3 
45*1 
JLf A Ll Q v_ U V V 
nitrogen... 
8.3 
8.6 
10.0 
8.6 
6.6 
8.1 
8.1 
8.9 
7 * 7 
9.1 
8.2 
10. 7 
7*5 
8.6 
7*3 
8.2 
Trypsin ni¬ 
trogen .... 
3 * 5 
3*5 
7.0 
7 -o 
7.0 
7.0 
7.0 
7.0 
14.0 
14.0 
4*7 
4*7 
9.4 
9.4 
9*4 
9.4 
a Results obtained at end of 26 and 168 hours, instead of 2 and 6 hours. 
b Results obtained at end of 7 and 24 hours, instead of 2 and 6 hours. 
