702 
Journal of Agricultural Research 
Vol. V, No. i S 
a little more rapidly than the beef. After 48 hours the digestion mix¬ 
ture of veal sample 11 smelled putrid. In addition to the amino nitro¬ 
gen liberated by the trypsin in this mixture non-amino nitrogen was 
transformed into amino nitrogen by the bacteria. This was indicated 
by the fact that after 48 hours' digestion amino nitrogen in veal sample 
11 was higher than the amount originally present in the meat. During 
the bacterial and tryptic action which followed, practically all of the 
nitrogen was transformed to amino nitrogen. The mixture of beef 
sample 11 did not smell putrid in this experiment. In experiment 34, 
which was a repetition of experiment 32 except that beef and veal 
samples 12 were used, both mixtures from these samples had become 
putrid, and in both, as the data in Table XI show, the amino nitrogen 
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Fig. 4.—Experiment 32. Curve showing the quantity (in milligrams) of amino nitrogen in 10 c. c. of diges¬ 
tion fluid; used 100 gm. of meat plus 2,000 c. c. of 0.5 per centsodium carbonate plus 4.000 gm. of trypsin 3. 
measured was greater in amount than that originally present in the 
meat. In some of the experiments putrefactive odors were not noticed, 
although looked for. 
The general conclusion drawn from the data of Table XII was the 
same as that drawn from Tables X and XI—namely, that mature beef and 
immature veal under the conditions of the experiments were digested 
by trypsin with equal speed. The slight differences noticed were re¬ 
garded as physiologically insignificant. 
In experiment 27 and its repetition, experiment 28, veal sample 9 was 
compared with skim-milk sample 2, with the same object as before, to 
ascertain whether the method would detect a difference in amino-nitrogen 
liberation where such a difference existed. In both experiments, up to 
and including the 11-hour determinations, amino nitrogen was liberated 
in the skim-milk digestion mixtures much more rapidly than in veal * 
sample 9. After this the results were somewhat irregular. 
