704 
Journal of Agricultural Research 
Vol. V, No. 15 
The principal object of the feeding experiments was to ascertain 
whether growth and reproduction were possible on a diet in which imma¬ 
ture veal was the sole source of nitrogen. The data of the above inves¬ 
tigators were used as a guide in planning the experiments. 
Diet.—T he cats' diet consisted of immature veal boiled for one to two 
hours, to which was added filtered butter fat, sodium chlorid, and calcium 
carbonate. The immature veal was obtained, as already described, from 
calves seven days old or less which were killed on the premises. When 
the meat was trimmed for feeding purposes, the lungs, heart, liver, kid¬ 
neys, and spleen, together with adherent bits of fat, gristle, etc., were 
included. For the purposes of the analytic work, digestion experiments, 
etc., the muscle tissue alone was wanted; for the feeding the intention 
was to include all parts of the veal that ordinarily are eaten. Thirty- 
four calves were fed to the cats. 
At suitable intervals of from four to seven days about 5 kgm. of veal 
were removed from the containers in cold storage. After being weighed 
the meat was cut into pieces about as large as ordinary sugar cubes, trans¬ 
ferred to an agate-ware kettle containing about 1 liter of hot water, and 
boiled for one to two hours. The object was to boil the meat in a small 
amount of water so that it would be convenient for feeding. 
Because of the low fat content of the veal, filtered butter fat was added 
after the boiled veal had cooled. This was obtained by melting several 
pounds of butter, allowing the water, casein, etc., to settle to the bottom 
of the containers, and pouring the supernatant fat through filter papers. 
The butter fat was kept in bottles in cold storage and used as required. 
According to Osborne and Mendel (1913, p. 424) butter fat contains no 
nitrogen. Funk and Macallum (1914) found traces of nitrogen in butter 
fat, which for the purposes of the present consideration of the diet may 
be disregarded. 
No analyses were made of the materials fed. In a few instances the 
carefully trimmed muscle tissue used for analyses, etc., was included in 
the veal diet. 
Following were the proportions of the various constituents of the diet: 
Immature veal. 1,300 gm. 
Filtered butter fat. 45 gm. , 
Calcium carbonate. 10 gm. 
Sodium chlorid. 10 gm. 
The last two constituents were the ordinary “chemically pure ana¬ 
lyzed" commercial products. The diet contained no roughage. The 
above proportions were calculated from the data of Osborne and Mendel 
(1911, p. 32, 80, 86). Potassium salts and phosphates were omitted, 
because these were thought to be present in the veal in sufficient amounts. 
After the veal had been boiled and the other materials added, the food 
was kept in an ice box close to the animals' cages. The gelatin present in 
the food caused the entire mass to become solid, so that there was no loss 
