Jan. 17, 1916 
Plenodomus Fuscomaculans 
729 
Table; IX.— Effect of aeration: Test with flasks of different sizes 
50.. . 
100 . 
250. . 
500.. 
1,000 
Size of flask. 
Number of 
pycnidia. 
Growth. 
C. c.. 
None. 
None. 
Boubtful. 
0 
Weak. 
O 
Fair, mycelium blackish. 
There were no checks in this experiment, but the behavior of this 
organism on filter paper had been so constant as to leave little doubt of 
repression of pycnidia having taken place, owing to the sealing of the 
flasks. 
A similar experiment was performed with a number of nutrient solu¬ 
tions, some of which were known to allow pycnidium production, and 
others of which were known to yield only strong growth. Ten c. c. of 
each solution were used. This experiment was done in duplicate and was 
carefully checked. Inoculation was made with small masses of mycelium. 
The flasks, after inoculation, were sealed and stood in strong diffuse light 
upon a table. Table X gives the summary of this experiment. 
- Table; X.— Effect of aeration: Tests with various nutrient solutions 
[Time, 1 month] 
Solution. 
Size of 
flask. 
Sealed. 
Check. 
Number of 
pycnidia. 
Growth. 
Number of 
pycnidia. 
Growth. 
C. c. 
f 900 
0 
Heavy mat. 
0 
Heavy mat. 
J 500 
0 
Heavy mat. 
0 
Heavy mat. 
I 125 
0 
Fair. 
0 
Heavy mat. 
l 50 
0 
Scant. 
0 
Heavy mat. 
9 °° 
O 
Scant. 
0 
Fair, white. 
s°° 
0 
Scant. 
0 
Fair, white. 
125 
0 
Scant. 
0 
Fair, white. 
So 
0 
None. 
0 
Fair, white. 
900 
O 
Very scant. 
0 
Weak. 
500 
O 
Very scant. 
0 
Weak. 
125 
0 
Very scant. 
0 
Weak. 
5 ° 
0 
None. 
0 
Weak. 
9 °° 
0 
Fair. 
0 
Heavy mat. 
500 
0 
Fair. 
0 
Heavy mat. 
125 
O 
Fair. 
0 
Heavy mat. 
l 5 o 
0 
Fair. 
0 
Heavy mat. 
Raulin solution 1 
(levulose sub¬ 
stituted for su¬ 
crose). 
Acid Box 2 solu¬ 
tion with. 1 c. c. 
of glycerin add¬ 
ed to each flask. 
Alkaline Box so¬ 
lution with 1 
c. c. of glycerin 
added to each 
flask. 
Raulin solution. 
1 Raulin solution: 1,500 parts of water; 70 parts of cane sugar (35 gm. levulose); 4 parts of tartaric acid; 
4 parts of ammonium nitrate; 0.6 part of ammonium phosphate; 0.4 part of magnesium carbonate; 0.6 
part of potassium carbonate; 0.25 part of ammonium sulphate; 0.07 part of zinc sulphate; 0.07 part of iron 
sulphate; 0.07 part of potassium silicate. 
2 Dox solution, etc. (Czapek): Distilled water (H2O), 3,000 c. c.; magnesium sulphate (MgSOd, 1.5 gm.; 
dibasic potassium phosphate (K2HPO4), 3.0 gm.; sodium chlorid (KC 1 ), 1.5 gm.; ferrous sulphate (FeSCh), 
0.03 gm.; with Potassium acid phosphate (KH2PO4), acid solution (Thom, 1910). 
17210°—16 - 2 
