830 
Journal of Agricultural Research 
Vol. V, No. 18 
in experiments 78 to 90—i. e., water, i,ooo c. c.; hydrochloric acid (sp. 
gr. 1.19), 10 c. c.; scale pepsin (U. S. P.), 2.5 gm.; sodium chlorid, 5 gm. 
A mixture of unfrozen meat from the six hogs was used in control exami¬ 
nations. As in the preceding experiments, five rats were fed meat from 
each can, following the same routine. Control animals were fed on June 
15 with unfrozen meat from the six hogs which had been kept several 
months (since March) in a cooler. Meat from each hog was fed to two 
rats, 20 gm. being given to each two rats, an average of 10 gm. per rat. 
In experiment 127, some meat from an artificially infested hog (the 
same hog from which meat was obtained in experiments 72 to 76) was 
inclosed in a half-pound tin can, which was placed in the center of a box 
28 by 19 by 6}4 inches containing about 100 pounds of pork trimmings. 
The box was placed in a freezer in Chicago, where it remained for 57 days, 
during which time the temperature as recorded by a thermometer 
afterwards compared with a standardized thermometer varied between 
io° and 13 0 F. (three readings daily). After removal from the freezer 
the box was allowed to thaw for two days. The can was then removed 
and sent to the Washington laboratory. The same routine as to the 
examination and feeding of test animals was followed as in experiments 
91 to 126. 
There were no satisfactory control test animals in experiment 127, as 
the rats fed as controls in experiments 72 to 76, which would have 
served as controls in this experiment, were not fed until nearly eight 
months had elapsed since the slaughter of the hog from which the meat 
was obtained. No infestation resulted in these animals; the trichinae 
were evidently all dead. Examination of some of the meat about six 
weeks later showed that the trichinae were dead and disintegrated. The 
trichinae, however, that were examined after artificial digestion of 
unfrozen meat from this hog as late as three weeks after slaughter 
appeared perfectly normal and were quite lively, and there is little doubt 
that control animals would have been infested if they had been fed early 
enough. 
See Tables I and II for the results of these experiments. 
