Feb. 7, 1916 
Agglutination Test for Bacterium abortus 
873 
Table I. —Test showing the appearance in milk of agglutinins for Bacterium abortus 
after the introduction into the cow's udder of a pure culture of Bact. abortus Bang a 
[Agglutination reaction at middle of milking, when various quantities of milk were added to test tubes 
containing bacterial suspension] 
« The +, —, and P signs used in all the tables refer to agglutination reaction in the corresponding tube. 
For instance, +++P— indicates that agglutination took place in the tubes containing o.i, 0.05, and 0.025 
c. c. of milk, {partial agglutination took place in the tube containing 0.01 c. c. of milk, and there was no 
agglutination in the tube containing 0.005 c - c - of milk. 
In all cases, unless otherwise stated, the milk was taken a little before what was estimated to be the 
middle of the milking. 
b 35 c. c. of a 48-hour broth culture of Bact. abortus introduced into right rear quarter. 
c Cow calved. Bull calf died oh Mar. 13,1915, owing to undigested curd. Reaction of blood of calf; 
—agglutination; +complement-fixation test. 
Table II gives the history of milk from a cOw with a record of fre¬ 
quent abortions. As shown in the table, the isolation of Bact. abortus 
from the milk and the results of guinea-pig inoculation prove the presence 
of this bacterium, as indicated by agglutination reactions. 
Table II. —History of milk from a cow with a record offrequent abortions a 
[Agglutination reaction at middle of milking, when various quantities of milk were added to test tubes 
containing bacterial suspension] 
Right rear quarter. 
Right ffont quarter. 
Left rear quarter. 
Left front quarter. 
d 
d 
d 
d 
d 
d 
d 
O 
y 
d 
d 
(J 
d 
d 
j 
y 
d 
d 
d 
d 
d 
d 
to 
d 
to 
d 
to 
d 
to 
0 
d 
to 
d 
IP 
d 
d 
lO 
d 
O 
l/> 
H 
to 
0 
8 
M 
O 
8 
H 
0 
8 
H 
O 
8 
H 
0 
0 
M 
O 
8 
M 
O 
q 
8 
w 
0 
a 
d 
d 
d 
d 
d 
6 
d 
d 
d 
d 
O 
d 
• d 
d 
d 
d 
0 
d 
d 
6 
+ 
+ 
+ 
— 
_ 
+ 
+ 
P 
__ 
— 
+ 
+ 
+ 
P 
_ 
+ 
+ 
+ 
+ 
— 
+ 
+ 
+ 
— 
— 
+ 
+ 
P 
— 
— 
+ 
+ 
— 
— 
— 
+ 
+ 
+ 
— 
— 
+ 
+ 
+ 
P 
— 
+ 
+ 
+ 
— 
— 
+ 
+ 
P 
— 
+ 
+ 
+ 
P 
— 
+ 
— 
— 
— 
— 
+ 
P 
— 
— 
— 
+ 
+ 
+ 
P 
+ 
P 
— 
— 
— 
+ 
+ 
+ 
+ 
+ 
P 
— 
+ 
+ 
+ 
P 
— 
+ 
+ 
+ 
P 
_ 
+ 
+ 
— 
— 
— 
+ 
— 
— 
— 
— 
+ 
+ 
— 
— 
— 
+ 
+ 
— 
— 
— 
+ 
+ 
— 
— 
— 
+ 
+ 
— 
— 
— 
+ 
+ 
+ 
— 
— 
+ 
+ 
+ 
— 
— 
+ 
+ 
P 
— 
— 
+ 
+ 
+ 
— 
— 
+ 
+ 
+ 
P 
— 
+ 
+ 
+ 
— 
— 
Date. 
1914. 
Jan. 10 * * 6 . 
Apr. 30. 
May 5. 
June 20. 
July 11. 
12 c . 
Aug. 10. 
28. 
Oct. 10. 
31. 
Nov. 19 d . 
a Known abortions: Dec., 1909; Jan., 1914. Jan., 1911, last living normal calf. Other records of abor¬ 
tions lost. 
& Isolated a pure culture of Bact. abortus direct from milk. 
c Guinea pigs inoculated intra-abdominally with milk from each quarter had typical Bact• abortus 
lesions when autopsies were performed eight weeks later. 
d Died; impaction of stomach. No lesions or abnormal conditions found in udder. 
