oct. is, 1917 Conglutination Test for the Diagnosis of Glanders 
7i 
Tabl3 V .—Titration of test serum 
Tube No. 
Serum. ^ 
Comple¬ 
ment. 6 
Inacti¬ 
vated cow 
serum, c 
Sheep- 
blood cor- 
puscles.d 
Result.« 
C. c. 
0 . OI 
C. c, 
0. I 
C. c . 
0.8 
C. c . 
O. I 
+ 
. 02 
. 1 
.8 
. I 
+ 
•03 
. 1 
.8 
. I 
+ 
4 . 
. 04 
. 1 
.8 
. I 
± 
•05 
. 1 
.8 
. I 
— 
6. 
. 06 
. 1 
.8 
. I 
" 
& Test serum. 
6 Fresh normal horse serum. 
c Inactivated cow serum diluted 2 : 78. 
d Five per cent suspension of sheep-blood corpuscles. 
« +=conglutination; ±=partial conglutination; —=no conglutination. 
With the above serum the largest amount not interfering with con¬ 
glutination (0.03 c. c.) would be used in the test. 
THE TEST PROPER 
Various investigators have recommended the use of different quanti¬ 
ties of the serum to be tested, ranging from 0.05 to 0.2 c. c. In this 
work 0.05 and 0.1 c. c. were found to be the most suitable. Four tubes 
are used to each serum sample to be tested, the first two tubes contain¬ 
ing 0.05 c. c. of serum (the first tube receiving antigen, the second being 
a control on the serum) and the third and fourth tubes containing 0.1 
c. c. (the third receiving antigen and the fourth serving as a control on 
0.1 c. c. of the serum). 
Table VI .—Results of the conglutination tests proper 
positive CASE 
Tube No. 
Suspected 
serum.** 
Antigen. 6 
Comple¬ 
ment. c 
Inactivated 
cow 
serum.** 
Sheep- 
blood cor¬ 
puscles. e 
Result./ 
I. 
C.c. 
0.05 
•05 
. I 
C.c. 
0. 08 
C. c. 
0. I 
C. c. 
0.8 
C. c. 
O. I 
2 g ... 
. I 
.8 
. 1 
+ 
.08 
. I 
.8 
. 1 
O ******** * * * * * * 
a a . 
. 1 
. I 
.8 
. 1 
+ 
9 * * * *.* * * * * 
NEGATIVE CASE 
c. 
•05 
•05 
. I 
.08 
. I 
. 8 
. I 
+ 
+ 
+ 
+ 
6 g . 
. I 
. 8 
. 1 
.08 
. I 
.8 
. 1 
$ g . 
. I 
, I 
.8 
. 1 
® Serum to be tested. 
6 Bacillus mallei extract, 
c Fresh normal horse serum. 
d Inactivated cow serum diluted 2 : 78. 
« Five per cent suspension of sheep red-blood cells. 
/ No conglutination^ positive case; +=conglutination=negative case. 
9 Tubes 2 and 4 and 6 and 8 are controls on the serum, and should not interfere with conglutination. 
