72 
Journal of Agricultural Research 
Vol. XI, No. 3 
The advantage in using the two quantities of serum is found in those 
serums which inhibit conglutination in quantities of o.i c. c. in the 
serum control, whereas no such inference is noted with the smaller 
quantity (0.05 c. c.), in which case a diagnosis can be made on this 
amount, thus saving the necessity of a retest with smaller amounts of 
serum. Also occasionally a paradoxical reaction will be observed in 
which a serum gives a positive reaction with 0.05 c. c. of serum and a 
negative reaction with 0.1 c. c. These cases, however, are rare. 
The results of the test are given in Table VI. 
RESULTS OR COMPLEMENT-FIXATION AND CONGLUTINATION TESTS ON 
HORSE SERUMS 
Four hundred and fifty-six samples of serums from horses and mules 
were tested by both the complement-fixation and conglutination tests. 
Unfortunately in a number of cases no post-mortem data could be 
obtained, but those samples in which positive results were obtained to 
both tests were in the majority of cases from animals giving a positive or 
suspicious reaction to the ophthalmic mallein and were undoubtedly 
affected with glanders (Table VII). 
Table VII. — Comparative tests of 341 serums from horses 
Number of 
samples. 
Comple¬ 
ment 
fixation. 
Congluti¬ 
nation. 
Post¬ 
mortem. 
Remarks. 
*34 
134 + 
134 + 
69+ 
Post-mortem report could not be obtained 
on other samples. 
174 
I 74 - 
174- 
3 
3 + 
3 “ 
2 + 
No post-mortem report on third obtainable. 
7 
7 " 
7 + 
1 + 
Post-mortem* data obtained in only 1 case, 
typical glanders lesions (chronic). 
S 
5 ? 
5 “ 
9 
9 ? 
9 + | 
No post-mortem report available. 
9 
9- 
9 ? ! 
Total number of samples tested= 341; positive to complement fixation= 137; positive to conglutination 
test=i$o; negative to complement fixation^ 190; negative to conglutination= 182; doubtful to comply 
ment fixation = 14; doubtful to conglutination^. 
As will be seen from Table VII, 134 samples gave positive results to 
both the complement-fixation and conglutination test, and 174 samples 
gave negative results to both tests. Three samples gave positive results 
to complement fixation and negative to conglutination. Post-mortem 
reports were obtained on two of these cases, typical glanders lesions being 
found, which were of an acute nature. In this connection it should be men¬ 
tioned that it has been determined by several investigators that in cases 
of recent infection the specific antibodies are detected sooner by comple¬ 
ment-fixation than by the conglutination test. 
Seven samples were negative to complement fixation and positive to 
conglutination, a post-mortem report being received in only one case, 
