98 
Journal of Agricultural Research 
Vol. XI, No. 4 
Table II.— Weight (in grains) and percentage gains or losses of the total air-dried mate¬ 
rial of Series I to V —Continued. 
PERCENTAGE GAIN OR LOSS 
County. 
Quantity 
of sul¬ 
phur 
added 
per acre. 
Soybeans. 
Clover 
hay. 
Oats. 
Alfalfa 
hay. 
Wheat. 
Hay. 
Grain. 
Straw. 
Grain. 
Straw. 
Grain. 
Eawrence .... 
Do 
Pounds . 
IOO 
200 
IOO 
200 
IOO 
200 
IOO 
200 
IOO 
200 
IOO 
200 
IOO 
200 
IOO 
200 
+ 6.9 
+ 3.4 
+ 19.O 
+ 4.6 
+8.3 
- 8.1 
+ -9 
+ 6.0 
+ 8.6 
+ 15*3 
- 4 -o 
+ 4. 8 
- 3-7 
+ i *3 
- 5 -o 
+ 6.4 
+ 5*9 
+16. 7 
+28. 7 
+ 7*5 
-4.7 
+ 8.4 
+14.4 
t 7,1 
+ 22. I 
+ 3*6 
+ 21 . 7 
- *7 
-8.0 
+ 2.2 
“ *9 
— 6. 0 
+ *9 
— 12. 7 
-8.0 
-1.4 
- 6. 5 
+ 34 -o 
+ 22 . O 
- 7 *o 
+27-3 
+ 9.6 
+ 33-3 
+ 3-2 
- 3 i -9 
+ 5 -x 
— 6. 2 
- 6.6 
+10. 7 
—20. 7 
- 5*2 
+ 1.6 
— 1. 6 
Warren . 
Do 
-19. I 
+ 3 *1 
- 8.5 
+ 10. 8 
—2. 2 
Mason . 
Do 
+ X.I 
+ 5*3 
—29.8 
+ 7 *i 
+ 3-9 
Muhlenburg.. 
Do 
-7.6 
—22.1 
- 17*3 
+ 4.2 
~ *03 
Barren. 
Do 
- 8-3 
+31.6 
- 3*6 
-2.7 
-8.8 
McCracken... 
Do 
+ 2.5 
— 14. 6 
H-n. 2 
+6.5 
Madison. 
Do 
+ 23 - 9 
+ 7.2 
“i *3 
— . 1 
+8. 4 
Jefferson. 
Do , 
+ l6. I 
+18.8 
+ 4-7 
- 7 *i 
+4.0 
EFFECT OF SUEPHUk ON THE TOTAE AND SUEPHATE-SUEPHUR CONTENT OF 
SOYBEANS, CEOVER, AND AEFAEFA 
The air-dried plants in Series I, II, and IV, were finely ground for this 
work, a composite sample being made of the triplicates in each case, and 
the soybean seeds were ground with the corresponding sample of hay. 
The total sulphur determinations were made by the sodium-peroxid 
method 1 and the sulphate sulphur was determined by the following 
procedure: 
Ten gm. of material were digested in 400 c. c. of water on the water 
bath for several hours, with frequent stirring. It stood overnight, and 
was made to 500 c. c. volume, first deducting the volume occupied by 
the sample. This was then filtered, and a 5-gm. aliquot used, to which 
was added 1 c. c. of hydrochloric acid (1:1) to precipitate the protein 
and organic matter. The whole was then heated on the water bath sev¬ 
eral hours longer, allowed to stand overnight, filtered, and washed. 
The filtrate was made slightly acid with hydrochloric acid, heated, and 
barium sulphate precipitated by adding, hot, 10 per cent barium-chlorid 
solution, and letting it stand overnight. 
The results obtained on the above samples are given in Table III. 
1 A slight modification was used, which is now the official method of the Association of Official Agri¬ 
cultural Chemists (Wiley, H. W., ed. Op. cit., p. 23.) 
