224 
Journal of Agricultural Research 
Vol, XI, No. 5 
transformed. In the old mycelium the ammonia content was very low 
and nitrate content relatively very high at the beginning of the experi¬ 
ment. After five days and two weeks the nitrate content was still high 
and the ammonia content relatively low. 
Table XIII .—Quantity (in parts per million) of nitrogen as ammonia and nitrates and 
N 2 in Agaricus tabularis rings, Akron, Colo., igi6 
Nitrogen in the form of 
ammonia. 
Nitrogen in the form of 
nitrates. 
Total nitrogen as N 2 .« 
Sample No. 
Location. 
Ring 6, June 21. 
vd 
C* i 
T 
r- 
bo 
m 
P 4 
00 
W 
i 
►—> 
« 
1 
l 
t—> 
cT 
bo 
1 
Ring 8, July 18. 
Ring 6, June 21. 
Ring 7, June 26. 
Ring B, June 28. 
M 
>> 
*3 
« 
I 
Ring 8, July 18. 
H 
« 
I 
VO 
bo 
I 
'd 
N 
4 ) 
§ 
1-1 
a ' 
Ring B, June 28. 
Ring 2, July 13. 
Ring 8, July 18. 
i 
Outside (see fig. 
13). 
2.9 
!. 4 
4*3 
7*9 
5-7 
8.6 
11.4 
11.4 
10. 7 
4*3 
1,190 
840 
770 
1,050 
1,190 
i 
.do. 
S-o 
4.0 
9-5 
7.6 
3-8 
4.0 
S-o 
3*8 
1.9 
.0 
I, 120 
840 
700 
910 
1,050 
2 
Youngest myce¬ 
lium . 
8.6 
10.0 
12.4 
6.4 
12.8 
14.3 
11.4 
21.4 
6.4 
11.4 
I, 120 
1,050 
770 
1 ) 05 ® 
910 
2 
.do. 
17.1 
•SO 
170. 
S-o 
11.0 
.0 
. 0 
.0 
4.0 
2.0 
1,190 
910 
77 o 
1,050 
910 
3 
Mycelium dens¬ 
est, driest. 
20.0 
17*3 
2.9 
27. 2 
75*7 
IS* 7 
11.4 
12.9 
7*9 
8.6 
910 
1,050 
700 
1,120 
1,050 
3 
.do. 
17.0 
21.0 
21. 6 
27.0 
22.9 
2.0 
4.0 
9 5 
11. 4 
S' 7 
770 
980 
840 
1,050 
980 
1,050 
4 
Below No. 3.. 
11. 4 
2.9 
4*3 
13-0 
11.4 
10.0 
12.9 
22. 9 
11. 4 
8.6 
1,050 
840 
770 
1 >330 
4 
.do. 
12.0 
3-0 
1. 0 
6.0 
6.0 
8.0 
1.0 
S*o 
6.0 
10.0 
1,050 
770 
770 
910 
I; 330 
S 
Old dying myce¬ 
lium. 
11.4 
5 * 7 
2.9 
6.4 
8.6 
8.6 
34 - 2 
22. 9 
32- 2 
22.9 
84O 
840 
700 
840 
910 
5 
.do. 
6.0 
17.1 
3 -o 
5 * 7 
14.0 
. 0 
26. 0 
10. 0 
30-5 
18.0 
84O 
770 
700 
840 
910 
6 
Inside. 
2.9 
2.9 
. 0 
3-6 
2.8 
10.0 
8.6 
1.4 
7.2 
7 * 1 
1,050 
770 
700 
910 
980 
6 
.do. 
5*7 
4-0 
.0 
6.6 
9*5 
. 0 
.0 
. 0 
4.0 
1.9 
980 
700 
700 
910 
840 
“ For these determinations the writers are indebted to Mr. H. W. Daudt, of the Bureau of Chemistry. 
The determinations were made by the Kjeldahl-Gunning-Arnold method. 
Under field conditions the growth of the mycelium is accompanied 
with the production of ammonia. In the older mycelium, on the other 
hand, the nitrification is rapid and most of the nitrogen is found as 
nitrates and only a comparatively small amount as ammonia. The 
nitrification and ammonification determinations are in accord with the 
determinations made from field samples. 
Table XIV. — Ammonification and nitrification (in parts per million) in Agaricus 
tabularis rings, Akron, Colo., May, iQi^ a 
Ammonification. 
Nitrification. 
Sample 
No. 
Location. 
Nitrogen as 
ammonia 
originally 
present in 
soil. 
Nitrogen as 
ammonia 
gained after 
5 days’ 
incubation 
with 0.2 
per cent of 
peptone. 
Nitrogen as 
ammonia 
gained after 
2 weeks' 
incubation 
with 0.2 
per cent of 
peptone, i 
| 
Nitrogen as 
i nitrate 
originally 
| present in 
1 soil. 
i 
(Nitrogen as 
j nitrate 
gained after 
5 days’ 
incubation 
with 0.2 
per cent of 
peptone. 
Nitrogen as 
nitrate 
gained after 
> 2 weeks' 
incubation 
with 0.2 
per cent of 
peptone. 
I 
Outside. 
3*3 
59-9 
55*9 
O. O 
5 -1 
I 5 - 3 
2 
Youngest myce¬ 
lium. 
I- 5 
34 * 7 
39*5 
9.0 
-1.4 
iS-8 
3 
Dense mycelium 
21 . O 
35-2 
40. 0 
21 . O 
• 5 
22. 8 
5 
Dying mycelium 
i- 5 
29. 0 
17. 1 
34*5 
28. $ 
ii7- 5 
a These determinations were made by Mr. R. C. Wright, of the Office of Soil Bacteriology, Bureau of 
Plant Industry, 
